8env: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older editNewer edit →
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
Line 1: Line 1:
'''Unreleased structure'''


The entry 8env is ON HOLD  until Paper Publication
==In situ cryo-EM structure of Pseudomonas phage E217 tail baseplate in C6 map==
<StructureSection load='8env' size='340' side='right'caption='[[8env]], [[Resolution|resolution]] 3.42&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8env]] is a 36 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_phage_vB_PaeM_E217 Pseudomonas phage vB_PaeM_E217]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8ENV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8ENV FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.42&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8env FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8env OCA], [https://pdbe.org/8env PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8env RCSB], [https://www.ebi.ac.uk/pdbsum/8env PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8env ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A2K8IA62_9CAUD A0A2K8IA62_9CAUD]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
E217 is a Pseudomonas phage used in an experimental cocktail to eradicate cystic fibrosis-associated Pseudomonas aeruginosa. Here, we describe the structure of the whole E217 virion before and after DNA ejection at 3.1 A and 4.5 A resolution, respectively, determined using cryogenic electron microscopy (cryo-EM). We identify and build de novo structures for 19 unique E217 gene products, resolve the tail genome-ejection machine in both extended and contracted states, and decipher the complete architecture of the baseplate formed by 66 polypeptide chains. We also determine that E217 recognizes the host O-antigen as a receptor, and we resolve the N-terminal portion of the O-antigen-binding tail fiber. We propose that E217 design principles presented in this paper are conserved across PB1-like Myoviridae phages of the Pbunavirus genus that encode a ~1.4 MDa baseplate, dramatically smaller than the coliphage T4.


Authors: Li, F., Cingolani, G., Hou, C.
High-resolution cryo-EM structure of the Pseudomonas bacteriophage E217.,Li F, Hou CD, Lokareddy RK, Yang R, Forti F, Briani F, Cingolani G Nat Commun. 2023 Jul 8;14(1):4052. doi: 10.1038/s41467-023-39756-z. PMID:37422479<ref>PMID:37422479</ref>


Description: In situ cryo-EM structure of Pseudomonas phage E217 tail baseplate in C6 map
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Cingolani, G]]
<div class="pdbe-citations 8env" style="background-color:#fffaf0;"></div>
[[Category: Li, F]]
== References ==
[[Category: Hou, C]]
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Pseudomonas phage vB_PaeM_E217]]
[[Category: Cingolani G]]
[[Category: Hou C]]
[[Category: Li F]]
Retrieved from "https://proteopedia.org/8env"