1ixf: Difference between revisions

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[[Image:1ixf.gif|left|200px]]
{{Seed}}
[[Image:1ixf.png|left|200px]]


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{{STRUCTURE_1ixf|  PDB=1ixf  |  SCENE=  }}  
{{STRUCTURE_1ixf|  PDB=1ixf  |  SCENE=  }}  


'''Crystal Structure of the K intermediate of bacteriorhodopsin'''
===Crystal Structure of the K intermediate of bacteriorhodopsin===




==Overview==
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Bacteriorhodopsin, the sole membrane protein of the purple membrane of Halobacterium salinarum, functions as a light-driven proton pump. A 3-D crystal of bacteriorhodopsin, which was prepared by the membrane fusion method, was used to investigate structural changes in the primary photoreaction. It was observed that when a frozen crystal was exposed to a low flux of X-ray radiation (5 x 10(14)photons mm(-2)), nearly half of the protein was converted into an orange species, exhibiting absorption peaks at 450 nm, 478 nm and 510 nm. The remainder retained the normal photochemical activity until Asp85 in the active site was decarboxlyated by a higher flux of X-ray radiation (10(16)photons mm(-2)). The procedure of diffraction measurement was improved so as to minimize the effects of the radiation damage and determine the true structural change associated with the primary photoreaction. Our structural model of the K intermediate indicates that the Schiff base linkage and the adjacent bonds in the polyene chain of retinal are largely twisted so that the Schiff base nitrogen atom still interacts with a water molecule located near Asp85. With respect to the other part of the protein, no appreciable displacement is induced in the primary photoreaction.
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{{ABSTRACT_PUBMED_12445782}}


==About this Structure==
==About this Structure==
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[[Category: Rsgi]]
[[Category: Rsgi]]
[[Category: Structural genomic]]
[[Category: Structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 14:10:19 2008''

Revision as of 11:10, 1 July 2008

File:1ixf.png

Template:STRUCTURE 1ixf

Crystal Structure of the K intermediate of bacteriorhodopsin

Template:ABSTRACT PUBMED 12445782

About this Structure

1IXF is a Single protein structure of sequence from Halobacterium salinarum. Full crystallographic information is available from OCA.

Reference

Specific damage induced by X-ray radiation and structural changes in the primary photoreaction of bacteriorhodopsin., Matsui Y, Sakai K, Murakami M, Shiro Y, Adachi S, Okumura H, Kouyama T, J Mol Biol. 2002 Nov 29;324(3):469-81. PMID:12445782

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