1iyy: Difference between revisions

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[[Image:1iyy.jpg|left|200px]]
{{Seed}}
[[Image:1iyy.png|left|200px]]


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{{STRUCTURE_1iyy|  PDB=1iyy  |  SCENE=  }}  
{{STRUCTURE_1iyy|  PDB=1iyy  |  SCENE=  }}  


'''NMR STRUCTURE OF Gln25-RIBONUCLEASE T1, 24 STRUCTURES'''
===NMR STRUCTURE OF Gln25-RIBONUCLEASE T1, 24 STRUCTURES===




==Overview==
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Ribonuclease (RNase) T1 is a guanyloribonuclease, having two isozymes in nature, Gln25- and Lys25-RNase T1. Between these two isozymes, there is no difference in catalytic activity and three-dimensional structure; however, Lys25-RNase T1 is slightly more stable than Gln25-RNase T1. Recently, it has been suggested that the existence of a salt bridge between Lys25 and Asp29/Glu31 in Lys25-RNase T1 contributes to the stability. To elucidate the effects of the replacement of Lys25 with a Gln on the conformation and microenvironments of RNase T1 in detail, the three-dimensional solution structure of Gln25-RNase T1 was determined by simulated-annealing calculations. As a result, the topology of the overall folding was shown to be very similar to that of the Lys25-isozyme except for some differences. In particular, there were two differences in the property of torsion angles of the two disulfide bonds and the conformations of the residues 11-13, 63-66, and 92-93. With regard to the residues 11-13, the lack of the above-mentioned salt bridge in Gln25-RNase T1 was thought to induce the conformational difference of this segment as compared with the Lys25-isozyme. Furthermore, it was proposed that the perturbation of this segment might transfer to the residues 92-93 via the two disulfide bonds.
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{{ABSTRACT_PUBMED_12974386}}


==About this Structure==
==About this Structure==
1IYY is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_oryzae Aspergillus oryzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IYY OCA].  
1IYY is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_oryzae Aspergillus oryzae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IYY OCA].  


==Reference==
==Reference==
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[[Category: Endoribonuclease]]
[[Category: Endoribonuclease]]
[[Category: Ribonuclease]]
[[Category: Ribonuclease]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:36:16 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 14:14:43 2008''

Revision as of 11:14, 1 July 2008

File:1iyy.png

Template:STRUCTURE 1iyy

NMR STRUCTURE OF Gln25-RIBONUCLEASE T1, 24 STRUCTURES

Template:ABSTRACT PUBMED 12974386

About this Structure

1IYY is a Single protein structure of sequence from Aspergillus oryzae. Full experimental information is available from OCA.

Reference

Determination of the NMR structure of Gln25-ribonuclease T1., Hatano K, Kojima M, Suzuki E, Tanokura M, Takahashi K, Biol Chem. 2003 Aug;384(8):1173-83. PMID:12974386

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