1j23: Difference between revisions

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[[Image:1j23.~~jpg~~|left|200px]]

[[Image:1j23.png|left|200px]]

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~~'''~~Crystal structure of archaeal XPF/Mus81 homolog, Hef from Pyrococcus furiosus, nuclease domain~~'''~~

===Crystal structure of archaeal XPF/Mus81 homolog, Hef from Pyrococcus furiosus, nuclease domain===

~~==Overview==~~

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The XPF/Rad1/Mus81-dependent nuclease family specifically cleaves branched structures generated during DNA repair, replication, and recombination, and is essential for maintaining genome stability. Here, we report the domain organization of an archaeal homolog (Hef) of this ~~family and the X-ray crystal structure of the middle domain~~, ~~with the nuclease activity. The nuclease domain architecture exhibits remarkable similarity to those of restriction endonucleases~~, ~~including~~ the ~~correspondence of the GDX(n)ERKX(3)D signature motif in Hef~~ to the ~~PDX~~(~~n)(E~~/D)~~XK motif in restriction enzymes~~. ~~This structural study also suggests that the XPF/Rad1/Mus81/ERCC1 proteins form a dimer through each interface of the nuclease domain and the helix~~-~~hairpin~~-~~helix domain. Simultaneous disruptions of both interfaces result in their dissociation into separate monomers, with strikingly reduced endonuclease activities.~~

The line below this paragraph, {{ABSTRACT_PUBMED_12679022}}, adds the Publication Abstract to the page

(as it appears on PubMed at http://www.pubmed.gov), where 12679022 is the PubMed ID number.

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==About this Structure==

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[[Category: Nishino, T.]]

[[Category: Structure-specific endonuclease]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Fri May~~ ~~2 20~~:42:46 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 14:23:22 2008''

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-[[Image:1j23.jpg|left|200px]]
+{{Seed}}
+[[Image:1j23.png|left|200px]]
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 {{STRUCTURE_1j23|  PDB=1j23  |  SCENE=  }}
-'''Crystal structure of archaeal XPF/Mus81 homolog, Hef from Pyrococcus furiosus, nuclease domain'''
+===Crystal structure of archaeal XPF/Mus81 homolog, Hef from Pyrococcus furiosus, nuclease domain===
-==Overview==
+<!--
-The XPF/Rad1/Mus81-dependent nuclease family specifically cleaves branched structures generated during DNA repair, replication, and recombination, and is essential for maintaining genome stability. Here, we report the domain organization of an archaeal homolog (Hef) of this family and the X-ray crystal structure of the middle domain, with the nuclease activity. The nuclease domain architecture exhibits remarkable similarity to those of restriction endonucleases, including the correspondence of the GDX(n)ERKX(3)D signature motif in Hef to the PDX(n)(E/D)XK motif in restriction enzymes. This structural study also suggests that the XPF/Rad1/Mus81/ERCC1 proteins form a dimer through each interface of the nuclease domain and the helix-hairpin-helix domain. Simultaneous disruptions of both interfaces result in their dissociation into separate monomers, with strikingly reduced endonuclease activities.
+The line below this paragraph, {{ABSTRACT_PUBMED_12679022}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 12679022 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_12679022}}
 ==About this Structure==
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 [[Category: Nishino, T.]]
 [[Category: Structure-specific endonuclease]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 20:42:46 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul  1 14:23:22 2008''