8h5b: Difference between revisions

Line 1:

~~'''Unreleased structure'''~~

The ~~entry~~ 8h5b is ~~ON HOLD until Paper~~ Publication

==The cryo-EM structure of nuclear transport receptor Kap114p complex with yeast TATA-box binding protein==

== Structural highlights ==

<table><tr><td colspan='2'>[[8h5b]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8H5B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8H5B FirstGlance]. <br>

</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.03Å</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8h5b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8h5b OCA], [https://pdbe.org/8h5b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8h5b RCSB], [https://www.ebi.ac.uk/pdbsum/8h5b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8h5b ProSAT]</span></td></tr>

</table>

== Function ==

[https://www.uniprot.org/uniprot/IMB5_YEAST IMB5_YEAST] Required for nuclear protein import and mediates docking of import substrate to distinct nucleoporins. Serves a receptor for nuclear localization signals. Mediates the nuclear import of TATA-binding protein (TBP) and of histones H2A and H2B.<ref>PMID:10535958</ref> <ref>PMID:11309407</ref>

== Publication Abstract from PubMed ==

The transcription factor TATA-box binding protein (TBP) modulates gene expression in nuclei. This process requires the involvement of nuclear transport receptors, collectively termed karyopherin-beta (Kap-beta) in yeast, and various regulatory factors. In previous studies we showed that Kap114p, a Kap-beta that mediates nuclear import of yeast TBP (yTBP), modulates yTBP-dependent transcription. However, how Kap114p associates with yTBP to exert its multifaceted functions has remained elusive. Here, we employ single-particle cryo-electron microscopy to determine the structure of Kap114p in complex with the core domain of yTBP (yTBP(C)). Remarkably, Kap114p wraps around the yTBP(C) N-terminal lobe, revealing a structure resembling transcriptional regulators in complex with TBP, suggesting convergent evolution of the two protein groups for a common function. We further demonstrate that Kap114p sequesters yTBP away from promoters, preventing a collapse of yTBP dynamics required for yeast responses to environmental stress. Hence, we demonstrate that nuclear transport receptors represent critical elements of the transcriptional regulatory network.

~~Authors: Hsia, K.C~~., Liao, ~~C.C.~~, Wang, C.H.~~, Wu, Y~~.M.

Structural convergence endows nuclear transport receptor Kap114p with a transcriptional repressor function toward TATA-binding protein.,Liao CC, Wang YS, Pi WC, Wang CH, Wu YM, Chen WY, Hsia KC Nat Commun. 2023 Sep 8;14(1):5518. doi: 10.1038/s41467-023-41206-9. PMID:37684250<ref>PMID:37684250</ref>

~~Description: The cryo~~-~~EM structure of nuclear transport receptor Kap114p complex with yeast TATA~~-~~box binding protein~~

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

[[Category: ~~Unreleased~~ Structures]]

</div>

[[Category: Hsia~~, K.C~~]]

[[Category: ~~Wu, Y.M~~]]

== References ==

[[Category: Wang~~, C.H~~]]

[[Category: ~~Liao, C.C~~]]

__TOC__

</StructureSection>

[[Category: Large Structures]]

[[Category: Saccharomyces cerevisiae S288C]]

[[Category: Hsia KC]]

[[Category: Liao CC]]

[[Category: Wang CH]]

[[Category: Wu YM]]

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 8h5b is ON HOLD  until Paper Publication
+==The cryo-EM structure of nuclear transport receptor Kap114p complex with yeast TATA-box binding protein==
+<StructureSection load='8h5b' size='340' side='right'caption='[[8h5b]], [[Resolution|resolution]] 4.03&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[8h5b]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8H5B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8H5B FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.03&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8h5b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8h5b OCA], [https://pdbe.org/8h5b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8h5b RCSB], [https://www.ebi.ac.uk/pdbsum/8h5b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8h5b ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/IMB5_YEAST IMB5_YEAST] Required for nuclear protein import and mediates docking of import substrate to distinct nucleoporins. Serves a receptor for nuclear localization signals. Mediates the nuclear import of TATA-binding protein (TBP) and of histones H2A and H2B.<ref>PMID:10535958</ref> <ref>PMID:11309407</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The transcription factor TATA-box binding protein (TBP) modulates gene expression in nuclei. This process requires the involvement of nuclear transport receptors, collectively termed karyopherin-beta (Kap-beta) in yeast, and various regulatory factors. In previous studies we showed that Kap114p, a Kap-beta that mediates nuclear import of yeast TBP (yTBP), modulates yTBP-dependent transcription. However, how Kap114p associates with yTBP to exert its multifaceted functions has remained elusive. Here, we employ single-particle cryo-electron microscopy to determine the structure of Kap114p in complex with the core domain of yTBP (yTBP(C)). Remarkably, Kap114p wraps around the yTBP(C) N-terminal lobe, revealing a structure resembling transcriptional regulators in complex with TBP, suggesting convergent evolution of the two protein groups for a common function. We further demonstrate that Kap114p sequesters yTBP away from promoters, preventing a collapse of yTBP dynamics required for yeast responses to environmental stress. Hence, we demonstrate that nuclear transport receptors represent critical elements of the transcriptional regulatory network.
-Authors: Hsia, K.C., Liao, C.C., Wang, C.H., Wu, Y.M.
+Structural convergence endows nuclear transport receptor Kap114p with a transcriptional repressor function toward TATA-binding protein.,Liao CC, Wang YS, Pi WC, Wang CH, Wu YM, Chen WY, Hsia KC Nat Commun. 2023 Sep 8;14(1):5518. doi: 10.1038/s41467-023-41206-9. PMID:37684250<ref>PMID:37684250</ref>
-Description: The cryo-EM structure of nuclear transport receptor Kap114p complex with yeast TATA-box binding protein
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Hsia, K.C]]
+<div class="pdbe-citations 8h5b" style="background-color:#fffaf0;"></div>
-[[Category: Wu, Y.M]]
+== References ==
-[[Category: Wang, C.H]]
+<references/>
-[[Category: Liao, C.C]]
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Saccharomyces cerevisiae S288C]]
+[[Category: Hsia KC]]
+[[Category: Liao CC]]
+[[Category: Wang CH]]
+[[Category: Wu YM]]