1j39: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older editNewer edit →
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,331,615 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,331,615 edits
No edit summary
Line 1: Line 1:
[[Image:1j39.jpg|left|200px]]
{{Seed}}
[[Image:1j39.png|left|200px]]


<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1j39|  PDB=1j39  |  SCENE=  }}  
{{STRUCTURE_1j39|  PDB=1j39  |  SCENE=  }}  


'''Crystal Structure of T4 phage BGT in complex with its UDP-glucose substrate'''
===Crystal Structure of T4 phage BGT in complex with its UDP-glucose substrate===




==Overview==
<!--  
T4 phage beta-glucosyltransferase (BGT) is an inverting glycosyltransferase (GT) that transfers glucose from uridine diphospho-glucose (UDP-glucose) to an acceptor modified DNA. BGT belongs to the GT-B structural superfamily, represented, so far, by five different inverting or retaining GT families. Here, we report three high-resolution X-ray structures of BGT and a point mutant solved in the presence of UDP-glucose. The two co-crystal structures of the D100A mutant show that, unlike the wild-type enzyme, this mutation prevents glucose hydrolysis. This strongly indicates that Asp100 is the catalytic base. We obtained the wild-type BGT-UDP-glucose complex by soaking substrate-free BGT crystals. Comparison with a previous structure of BGT solved in the presence of the donor product UDP and an acceptor analogue provides the first model of an inverting GT-B enzyme in which both the donor and acceptor substrates are bound to the active site. The structural analyses support the in-line displacement reaction mechanism previously proposed, locate residues involved in donor substrate specificity and identify the catalytic base.
The line below this paragraph, {{ABSTRACT_PUBMED_12860129}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 12860129 is the PubMed ID number.
-->
{{ABSTRACT_PUBMED_12860129}}


==About this Structure==
==About this Structure==
Line 28: Line 32:
[[Category: Gt-b]]
[[Category: Gt-b]]
[[Category: Udp-glucose]]
[[Category: Udp-glucose]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:45:07 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 14:26:11 2008''

Revision as of 11:26, 1 July 2008

File:1j39.png

Template:STRUCTURE 1j39

Crystal Structure of T4 phage BGT in complex with its UDP-glucose substrate

Template:ABSTRACT PUBMED 12860129

About this Structure

1J39 is a Single protein structure of sequence from Enterobacteria phage t4. Full crystallographic information is available from OCA.

Reference

Crystal structures of the T4 phage beta-glucosyltransferase and the D100A mutant in complex with UDP-glucose: glucose binding and identification of the catalytic base for a direct displacement mechanism., Lariviere L, Gueguen-Chaignon V, Morera S, J Mol Biol. 2003 Jul 25;330(5):1077-86. PMID:12860129

Page seeded by OCA on Tue Jul 1 14:26:11 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "https://proteopedia.org/index.php?title=1j39&oldid=614752"