Sandbox Reserved 1741: Difference between revisions
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== Relevance == | == Relevance == | ||
The finding of HIV-1 RT was monumental for offering treatment to individuals living with HIV-1. After its discovery, the development of nucleoside reverse transcriptase inhibitors (NRTIs) and non-nucleoside reverse transcriptase inhibitors (NNRTIs), which are responsible for inhibition of different mechanisms of reverse transcription (6). | The finding of HIV-1 RT was monumental for offering treatment to individuals living with HIV-1. After its discovery, the development of nucleoside reverse transcriptase inhibitors (NRTIs) and non-nucleoside reverse transcriptase inhibitors (NNRTIs), which are responsible for inhibition of different mechanisms of reverse transcription. Once the NNRTs come in contact with the RT, the process is stoped and the proviral DNA remains incomplete (6). | ||
"Of the 26 drugs currently approved to treat HIV-1 infections, 14 are RT inhibitors" (4). | "Of the 26 drugs currently approved to treat HIV-1 infections, 14 are RT inhibitors" (4). | ||
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== Structural highlights == | == Structural highlights == | ||
HIV reverse transcriptase is a heterodimer with chains of 66 kDA and 51 kDA. P66 chain is proteolytically processed by HIV protease to form the smaller unit of the heterodimer. HIV-1 reverse transcriptase produces a homodimer p66/p66 and a heterodimer p66/p51 when expressed in bacteria. Ribonucleases H (RNase H) are enzymes that cleavage the RNA of DNA/RNA hybrid. In reverse transcriptase the hydrolysis of an RNA strand in a DNA/RNA hybrid generates 5' phosphate and 3’ hydroxyl ends. | HIV reverse transcriptase is a heterodimer with chains of 66 kDA and 51 kDA. P66 chain is proteolytically processed by HIV protease to form the smaller unit of the heterodimer. HIV-1 reverse transcriptase produces a homodimer p66/p66 and a heterodimer p66/p51 when expressed in bacteria. Ribonucleases H (RNase H) are enzymes that cleavage the RNA of DNA/RNA hybrid. In reverse transcriptase the hydrolysis of an RNA strand in a DNA/RNA hybrid generates 5' phosphate and 3’ hydroxyl ends. | ||
== References == | == References == | ||
