1hne: Difference between revisions
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New page: left|200px<br /> <applet load="1hne" size="450" color="white" frame="true" align="right" spinBox="true" caption="1hne, resolution 1.84Å" /> '''STRUCTURE OF HUMAN ... |
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[[Image:1hne.gif|left|200px]]<br /> | [[Image:1hne.gif|left|200px]]<br /><applet load="1hne" size="350" color="white" frame="true" align="right" spinBox="true" | ||
<applet load="1hne" size=" | |||
caption="1hne, resolution 1.84Å" /> | caption="1hne, resolution 1.84Å" /> | ||
'''STRUCTURE OF HUMAN NEUTROPHIL ELASTASE IN COMPLEX WITH A PEPTIDE CHLOROMETHYL KETONE INHIBITOR AT 1.84-ANGSTROMS RESOLUTION'''<br /> | '''STRUCTURE OF HUMAN NEUTROPHIL ELASTASE IN COMPLEX WITH A PEPTIDE CHLOROMETHYL KETONE INHIBITOR AT 1.84-ANGSTROMS RESOLUTION'''<br /> | ||
==Overview== | ==Overview== | ||
Human neutrophil elastase (HNE) has been implicated as a major contributor | Human neutrophil elastase (HNE) has been implicated as a major contributor to tissue destruction in various disease states, including emphysema. The structure of HNE, at neutral pH, in complex with methoxysuccinyl-Ala-Ala-Pro-Ala chloromethyl ketone (MSACK), has been solved and refined to an R factor of 16.4% at 1.84-A resolution. Results are consistent with the currently accepted mechanism of peptide chloromethyl ketone inhibition of serine proteases, in that MSACK cross-links the catalytic residues His-57 and Ser-195. The structure of the HNE-MSACK complex is compared with that of porcine pancreatic elastase in complex with L-647,957, a beta-lactam inhibitor of both elastases. The distribution of positively charged residues on HNE is highly asymmetric and may play a role in its specific association with the underlying negatively charged proteoglycan matrix of the neutrophil granules in which the enzyme is stored. | ||
==Disease== | ==Disease== | ||
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==About this Structure== | ==About this Structure== | ||
1HNE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Active as [http://en.wikipedia.org/wiki/Leukocyte_elastase Leukocyte elastase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.37 3.4.21.37] Full crystallographic information is available from [http:// | 1HNE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Active as [http://en.wikipedia.org/wiki/Leukocyte_elastase Leukocyte elastase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.37 3.4.21.37] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HNE OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Leukocyte elastase]] | [[Category: Leukocyte elastase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Dorn, C | [[Category: Dorn, C P.]] | ||
[[Category: Fluder, E | [[Category: Fluder, E M.]] | ||
[[Category: Hoogsteen, K.]] | [[Category: Hoogsteen, K.]] | ||
[[Category: Lin, T | [[Category: Lin, T Y.]] | ||
[[Category: Mckeever, B | [[Category: Mckeever, B M.]] | ||
[[Category: Navia, M | [[Category: Navia, M A.]] | ||
[[Category: Springer, J | [[Category: Springer, J P.]] | ||
[[Category: Williams, H | [[Category: Williams, H R.]] | ||
[[Category: hydrolase(serine proteinase)]] | [[Category: hydrolase(serine proteinase)]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:02:58 2008'' | ||
