1hry: Difference between revisions

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[[Image:1hry.gif|left|200px]]<br />

[[Image:1hry.gif|left|200px]]<br /><applet load="1hry" size="350" color="white" frame="true" align="right" spinBox="true"

<applet load="1hry" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

caption="1hry" />

'''THE 3D STRUCTURE OF THE HUMAN SRY-DNA COMPLEX SOLVED BY MULTID-DIMENSIONAL HETERONUCLEAR-EDITED AND-FILTERED NMR'''<br />

==Overview==

The solution structure of the specific complex between the high mobility, group (HMG) domain of SRY (hSRY-HMG), the protein encoded by the human, testis-determining gene, and its DNA target site in the promoter of the, mullerian inhibitory substance gene has been determined by, multidimensional NMR spectroscopy. hSRY-HMG has a twisted L shape that, presents a concave surface (made up of three helices and the N- and, C-terminal strands) to the DNA for sequence-specific recognition. Binding, of hSRY-HMG to its specific target site occurs exclusively in the minor, groove and induces a large conformational change in the DNA. The DNA in, the complex has an overall 70 degrees-80 degrees bend and is helically, unwound relative to classical A- and B-DNA. The structure of the complex, reveals the origin of sequence-specific binding within the HMG-1/HMG-2, family and provides a framework for understanding the effects of point, mutations that cause 46X,Y sex reversal at the atomic level.

The solution structure of the specific complex between the high mobility group (HMG) domain of SRY (hSRY-HMG), the protein encoded by the human testis-determining gene, and its DNA target site in the promoter of the mullerian inhibitory substance gene has been determined by multidimensional NMR spectroscopy. hSRY-HMG has a twisted L shape that presents a concave surface (made up of three helices and the N- and C-terminal strands) to the DNA for sequence-specific recognition. Binding of hSRY-HMG to its specific target site occurs exclusively in the minor groove and induces a large conformational change in the DNA. The DNA in the complex has an overall 70 degrees-80 degrees bend and is helically unwound relative to classical A- and B-DNA. The structure of the complex reveals the origin of sequence-specific binding within the HMG-1/HMG-2 family and provides a framework for understanding the effects of point mutations that cause 46X,Y sex reversal at the atomic level.

==Disease==

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==About this Structure==

1HRY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1HRY OCA].

1HRY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HRY OCA].

==Reference==

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[[Category: Homo sapiens]]

[[Category: Single protein]]

[[Category: Clore, G.M.]]

[[Category: Clore, G M.]]

[[Category: Gronenborn, A.M.]]

[[Category: Gronenborn, A M.]]

[[Category: Huth, J.R.]]

[[Category: Huth, J R.]]

[[Category: Werner, M.H.]]

[[Category: Werner, M H.]]

[[Category: dna]]

[[Category: dna-binding protein]]

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[[Category: sry]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Mon Nov 12 17~~:21:~~40 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:04:15 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1hry.gif|left|200px]]<br />
+[[Image:1hry.gif|left|200px]]<br /><applet load="1hry" size="350" color="white" frame="true" align="right" spinBox="true"
-<applet load="1hry" size="450" color="white" frame="true" align="right" spinBox="true"
 caption="1hry" />
 '''THE 3D STRUCTURE OF THE HUMAN SRY-DNA COMPLEX SOLVED BY MULTID-DIMENSIONAL HETERONUCLEAR-EDITED AND-FILTERED NMR'''<br />
 ==Overview==
-The solution structure of the specific complex between the high mobility, group (HMG) domain of SRY (hSRY-HMG), the protein encoded by the human, testis-determining gene, and its DNA target site in the promoter of the, mullerian inhibitory substance gene has been determined by, multidimensional NMR spectroscopy. hSRY-HMG has a twisted L shape that, presents a concave surface (made up of three helices and the N- and, C-terminal strands) to the DNA for sequence-specific recognition. Binding, of hSRY-HMG to its specific target site occurs exclusively in the minor, groove and induces a large conformational change in the DNA. The DNA in, the complex has an overall 70 degrees-80 degrees bend and is helically, unwound relative to classical A- and B-DNA. The structure of the complex, reveals the origin of sequence-specific binding within the HMG-1/HMG-2, family and provides a framework for understanding the effects of point, mutations that cause 46X,Y sex reversal at the atomic level.
+The solution structure of the specific complex between the high mobility group (HMG) domain of SRY (hSRY-HMG), the protein encoded by the human testis-determining gene, and its DNA target site in the promoter of the mullerian inhibitory substance gene has been determined by multidimensional NMR spectroscopy. hSRY-HMG has a twisted L shape that presents a concave surface (made up of three helices and the N- and C-terminal strands) to the DNA for sequence-specific recognition. Binding of hSRY-HMG to its specific target site occurs exclusively in the minor groove and induces a large conformational change in the DNA. The DNA in the complex has an overall 70 degrees-80 degrees bend and is helically unwound relative to classical A- and B-DNA. The structure of the complex reveals the origin of sequence-specific binding within the HMG-1/HMG-2 family and provides a framework for understanding the effects of point mutations that cause 46X,Y sex reversal at the atomic level.
 ==Disease==
@@ Line 11: / Line 10: @@
 ==About this Structure==
-HRY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1HRY OCA].
+HRY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HRY OCA].
 ==Reference==
@@ Line 17: / Line 16: @@
 [[Category: Homo sapiens]]
 [[Category: Single protein]]
-[[Category: Clore, G.M.]]
+[[Category: Clore, G M.]]
-[[Category: Gronenborn, A.M.]]
+[[Category: Gronenborn, A M.]]
-[[Category: Huth, J.R.]]
+[[Category: Huth, J R.]]
-[[Category: Werner, M.H.]]
+[[Category: Werner, M H.]]
 [[Category: dna]]
 [[Category: dna-binding protein]]
@@ Line 26: / Line 25: @@
 [[Category: sry]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 17:21:40 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:04:15 2008''