|
|
| Line 1: |
Line 1: |
| [[Image:1jba.jpg|left|200px]] | | {{Seed}} |
| | [[Image:1jba.png|left|200px]] |
|
| |
|
| <!-- | | <!-- |
| Line 9: |
Line 10: |
| {{STRUCTURE_1jba| PDB=1jba | SCENE= }} | | {{STRUCTURE_1jba| PDB=1jba | SCENE= }} |
|
| |
|
| '''UNMYRISTOYLATED GCAP-2 WITH THREE CALCIUM IONS BOUND'''
| | ===UNMYRISTOYLATED GCAP-2 WITH THREE CALCIUM IONS BOUND=== |
|
| |
|
|
| |
|
| ==Overview==
| | <!-- |
| Guanylyl cyclase activating protein-2 (GCAP-2) is a Ca2+-sensitive regulator of phototransduction in retinal photoreceptor cells. GCAP-2 activates retinal guanylyl cyclases at low Ca2+ concentration (<100 nM) and inhibits them at high Ca2+ (>500 nM). The light-induced lowering of the Ca2+ level from approximately 500 nM in the dark to approximately 50 nM following illumination is known to play a key role in visual recovery and adaptation. We report here the three-dimensional structure of unmyristoylated GCAP-2 with three bound Ca2+ ions as determined by nuclear magnetic resonance spectroscopy of recombinant, isotopically labeled protein. GCAP-2 contains four EF-hand motifs arranged in a compact tandem array like that seen previously in recoverin. The root mean square deviation of the main chain atoms in the EF-hand regions is 2.2 A in comparing the Ca2+-bound structures of GCAP-2 and recoverin. EF-1, as in recoverin, does not bind calcium because it contains a disabling Cys-Pro sequence. GCAP-2 differs from recoverin in that the calcium ion binds to EF-4 in addition to EF-2 and EF-3. A prominent exposed patch of hydrophobic residues formed by EF-1 and EF-2 (Leu24, Trp27, Phe31, Phe45, Phe48, Phe49, Tyr81, Val82, Leu85, and Leu89) may serve as a target-binding site for the transmission of calcium signals to guanylyl cyclase.
| | The line below this paragraph, {{ABSTRACT_PUBMED_10383444}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 10383444 is the PubMed ID number. |
| | --> |
| | {{ABSTRACT_PUBMED_10383444}} |
|
| |
|
| ==About this Structure== | | ==About this Structure== |
| 1JBA is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JBA OCA]. | | 1JBA is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JBA OCA]. |
|
| |
|
| ==Reference== | | ==Reference== |
| Line 31: |
Line 35: |
| [[Category: Ef-hand]] | | [[Category: Ef-hand]] |
| [[Category: Guanylyl cyclase regulation]] | | [[Category: Guanylyl cyclase regulation]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:00:36 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 19:57:34 2008'' |
