1jo6: Difference between revisions

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[[Image:1jo6.gif|left|200px]]
{{Seed}}
[[Image:1jo6.png|left|200px]]


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{{STRUCTURE_1jo6|  PDB=1jo6  |  SCENE=  }}  
{{STRUCTURE_1jo6|  PDB=1jo6  |  SCENE=  }}  


'''Solution structure of the cytoplasmic N-terminus of the BK beta-subunit KCNMB2'''
===Solution structure of the cytoplasmic N-terminus of the BK beta-subunit KCNMB2===




==Overview==
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The auxiliary beta-subunit KCNMB2 (beta(2)) endows the non-inactivating large conductance Ca(2+)- and voltage-dependent potassium (BK) channel with fast inactivation. This process is mediated by the N terminus of KCNMB2 and closely resembles the "ball-and-chain"-type inactivation observed in voltage-gated potassium channels. Here we investigated the solution structure and function of the KCNMB2 N terminus (amino acids 1-45, BKbeta(2)N) using NMR spectroscopy and patch clamp recordings. BKbeta(2)N completely inactivated BK channels when applied to the cytoplasmic side; its interaction with the BK alpha-subunit is characterized by a particularly slow dissociation rate and an affinity in the upper nanomolar range. The BKbeta(2)N structure comprises two domains connected by a flexible linker: the pore-blocking "ball domain" (formed by residues 1-17) and the "chain domain" (between residues 20-45) linking it to the membrane segment of KCNMB2. The ball domain is made up of a flexible N terminus anchored at a well ordered loop-helix motif. The chain domain consists of a 4-turn helix with an unfolded linker at its C terminus. These structural properties explain the functional characteristics of BKbeta(2)N-mediated inactivation.
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{{ABSTRACT_PUBMED_11517232}}


==About this Structure==
==About this Structure==
1JO6 is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JO6 OCA].  
1JO6 is a [[Single protein]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JO6 OCA].  


==Reference==
==Reference==
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[[Category: Helix]]
[[Category: Helix]]
[[Category: Ion channel]]
[[Category: Ion channel]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:29:34 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 20:32:39 2008''

Revision as of 17:32, 1 July 2008

File:1jo6.png

Template:STRUCTURE 1jo6

Solution structure of the cytoplasmic N-terminus of the BK beta-subunit KCNMB2

Template:ABSTRACT PUBMED 11517232

About this Structure

1JO6 is a Single protein structure. Full experimental information is available from OCA.

Reference

NMR structure of the "ball-and-chain" domain of KCNMB2, the beta 2-subunit of large conductance Ca2+- and voltage-activated potassium channels., Bentrop D, Beyermann M, Wissmann R, Fakler B, J Biol Chem. 2001 Nov 9;276(45):42116-21. Epub 2001 Aug 21. PMID:11517232

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