1js2: Difference between revisions

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[[Image:1js2.gif|left|200px]]
{{Seed}}
[[Image:1js2.png|left|200px]]


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{{STRUCTURE_1js2|  PDB=1js2  |  SCENE=  }}  
{{STRUCTURE_1js2|  PDB=1js2  |  SCENE=  }}  


'''Crystal structure of C77S HiPIP: a serine ligated [4Fe-4S} cluster'''
===Crystal structure of C77S HiPIP: a serine ligated [4Fe-4S} cluster===




==Overview==
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The crystal structure of Chromatium vinosum C77S HiPIP has been determined and is compared with that of wild type. This is the first reported crystal structure of a Ser ligated [4Fe-4S] cluster and reveals a 0.11 A shortening of the Fe-O bond (relative to Fe-S), but only minor structural alterations of the overall tertiary structure. Coordination changes are corroborated by resonance Raman spectroscopy. Comparison of the crystal and solution structures for HiPIPs identifies Phe48 as the main controller of solvent access to the Fe-S cluster; however, there is no significant change in cluster solvation of the C77S mutant relative to WT HiPIP. Ser ligation ultimately results in decreased cluster stability due to increased sensitivity to proton mediated degradation.
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{{ABSTRACT_PUBMED_11802718}}


==About this Structure==
==About this Structure==
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[[Category: Sundaralingam, M.]]
[[Category: Sundaralingam, M.]]
[[Category: Xiong, Y.]]
[[Category: Xiong, Y.]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:48:23 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 20:43:07 2008''

Revision as of 17:43, 1 July 2008

File:1js2.png

Template:STRUCTURE 1js2

Crystal structure of C77S HiPIP: a serine ligated [4Fe-4S} cluster

Template:ABSTRACT PUBMED 11802718

About this Structure

1JS2 is a Single protein structure of sequence from Allochromatium vinosum. Full crystallographic information is available from OCA.

Reference

Crystal structure and stability studies of C77S HiPIP: a serine ligated [4Fe-4S] cluster., Mansy SS, Xiong Y, Hemann C, Hille R, Sundaralingam M, Cowan JA, Biochemistry. 2002 Jan 29;41(4):1195-201. PMID:11802718

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