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| [[Image:1jtm.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_1jtm| PDB=1jtm | SCENE= }} | | {{STRUCTURE_1jtm| PDB=1jtm | SCENE= }} |
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| '''Alternative Structures of a Sequence Extended T4 Lysozyme Show that the Highly Conserved Beta-Sheet has Weak Intrinsic Folding Propensity'''
| | ===Alternative Structures of a Sequence Extended T4 Lysozyme Show that the Highly Conserved Beta-Sheet has Weak Intrinsic Folding Propensity=== |
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| ==Overview==
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| Residues 24 to 35 of T4 lysozyme correspond to the second and third strands of a region of beta-sheet that is highly conserved in all known lysozyme and chitinase structures. To evaluate the intrinsic propensity of these amino acid residues to form a defined structure they were added at the C terminus of the native protein, together with a dipeptide linker. Two crystal structures of this active, mutant protein were obtained, to 1.9A and 2.3A resolution, respectively. Even though the crystal conditions are similar, the appended sequence adopts very different secondary structures. In one case it is weakly structured and appears to extend through the active-site cleft, perhaps in part adding an extra strand to the original beta-sheet. In the other crystal form the extension is largely alpha-helical. The formation of these alternative structures shows that the sequence does not have a strong intrinsic propensity to form a unique fold (either beta-sheet or otherwise). The results also suggest that structural conservation during evolution does not necessarily depend on sequence conservation or the conservation of folding propensity.
| | The line below this paragraph, {{ABSTRACT_PUBMED_11884133}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 11884133 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_11884133}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Sequence duplication]] | | [[Category: Sequence duplication]] |
| [[Category: Sequence repeat]] | | [[Category: Sequence repeat]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:54:15 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 20:47:00 2008'' |
