1imx: Difference between revisions
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New page: left|200px<br /> <applet load="1imx" size="450" color="white" frame="true" align="right" spinBox="true" caption="1imx, resolution 1.82Å" /> '''1.8 Angstrom crysta... |
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[[Image:1imx.gif|left|200px]]<br /> | [[Image:1imx.gif|left|200px]]<br /><applet load="1imx" size="350" color="white" frame="true" align="right" spinBox="true" | ||
<applet load="1imx" size=" | |||
caption="1imx, resolution 1.82Å" /> | caption="1imx, resolution 1.82Å" /> | ||
'''1.8 Angstrom crystal structure of IGF-1'''<br /> | '''1.8 Angstrom crystal structure of IGF-1'''<br /> | ||
==Overview== | ==Overview== | ||
Despite efforts spanning considerably more than a decade, a | Despite efforts spanning considerably more than a decade, a high-resolution view of the family of proteins known as insulin-like growth factors (IGFs) has remained elusive. IGF-1 consists of three helical segments which are connected by a 12-residue linker known as the C-region. NMR studies of members of this family reveal a dynamic structure with a topology resembling insulin but little structural definition in the C-region. We have crystallized IGF-1 in the presence of the detergent deoxy big CHAPS, and determined its structure at 1.8 A resolution by multiwavelength anomalous diffraction, exploiting the anomalous scattering of a single bromide ion and six of the seven sulfur atoms of IGF-1. The structure reveals a well-defined conformation for much of the C-region, which extends away from the core of IGF-1 and has residues known to be involved in receptor binding prominently displayed in a type II beta-turn. In the crystal, these residues form a dimer interface, but analytical ultracentrifugation experiments demonstrate that at physiological concentrations IGF-1 is monomeric. A single detergent molecule contacts residues known to be important for IGF-1 binding protein (IGFBP) interactions. Biophysical and biochemical data show that the detergent binds to IGF-1 specifically and blocks binding of IGFBP-1 and IGFBP-3. | ||
==Disease== | ==Disease== | ||
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==About this Structure== | ==About this Structure== | ||
1IMX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with BR and CPQ as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | 1IMX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=BR:'>BR</scene> and <scene name='pdbligand=CPQ:'>CPQ</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IMX OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Deshayes, K | [[Category: Deshayes, K D.]] | ||
[[Category: Liu, J.]] | [[Category: Liu, J.]] | ||
[[Category: Schaffer, M | [[Category: Schaffer, M L.]] | ||
[[Category: Skelton, N | [[Category: Skelton, N J.]] | ||
[[Category: Ultsch, M.]] | [[Category: Ultsch, M.]] | ||
[[Category: Vajdos, F | [[Category: Vajdos, F F.]] | ||
[[Category: Vos, A | [[Category: Vos, A M.de.]] | ||
[[Category: BR]] | [[Category: BR]] | ||
[[Category: CPQ]] | [[Category: CPQ]] | ||
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[[Category: insulin/relaxin]] | [[Category: insulin/relaxin]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:13:34 2008'' | ||
