1jw0: Difference between revisions

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[[Image:1jw0.gif|left|200px]]
{{Seed}}
[[Image:1jw0.png|left|200px]]


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{{STRUCTURE_1jw0|  PDB=1jw0  |  SCENE=  }}  
{{STRUCTURE_1jw0|  PDB=1jw0  |  SCENE=  }}  


'''Structure of cephalosporin acylase in complex with glutarate'''
===Structure of cephalosporin acylase in complex with glutarate===




==Overview==
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BACKGROUND: Semisynthetic cephalosporins are primarily synthesized from 7-aminocephalosporanic acid (7-ACA), which is obtained by environmentally toxic chemical deacylation of cephalosporin C (CPC). Thus, the enzymatic conversion of CPC to 7-ACA by cephalosporin acylase (CA) would be of great interest. However, CAs use glutaryl-7-ACA (GL-7-ACA) as a primary substrate and the enzyme has low turnover rates for CPC. RESULTS: The binary complex structures of CA with GL-7-ACA and glutarate (the side-chain of GL-7-ACA) show extensive interactions between the glutaryl moiety of GL-7-ACA and the seven residues that form the side-chain pocket. These interactions explain why the D-alpha-aminoadipyl side-chain of CPC yields a poorer substrate than GL-7-ACA. CONCLUSIONS: This understanding of the nature of substrate specificity may be useful in the design of an enzyme with an improved performance for the conversion of CPC to 7-ACA. Additionally, the catalytic mechanism of the deacylation reaction was revealed by the ligand bound structures.
The line below this paragraph, {{ABSTRACT_PUBMED_11755403}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 11755403 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11755403}}


==About this Structure==
==About this Structure==
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[[Category: Glutarate]]
[[Category: Glutarate]]
[[Category: Glutaryll-7-aca]]
[[Category: Glutaryll-7-aca]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:59:40 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 21:03:01 2008''

Revision as of 18:03, 1 July 2008

File:1jw0.png

Template:STRUCTURE 1jw0

Structure of cephalosporin acylase in complex with glutarate

Template:ABSTRACT PUBMED 11755403

About this Structure

1JW0 is a Protein complex structure of sequences from Brevundimonas diminuta. Full crystallographic information is available from OCA.

Reference

Structure of cephalosporin acylase in complex with glutaryl-7-aminocephalosporanic acid and glutarate: insight into the basis of its substrate specificity., Kim Y, Hol WG, Chem Biol. 2001 Dec;8(12):1253-64. PMID:11755403

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