1k23: Difference between revisions

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[[Image:1k23.jpg|left|200px]]
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[[Image:1k23.png|left|200px]]


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{{STRUCTURE_1k23|  PDB=1k23  |  SCENE=  }}  
{{STRUCTURE_1k23|  PDB=1k23  |  SCENE=  }}  


'''Inorganic Pyrophosphatase (Family II) from Bacillus subtilis'''
===Inorganic Pyrophosphatase (Family II) from Bacillus subtilis===




==Overview==
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Recently, a new class of soluble inorganic pyrophosphatase (type-C PPase) has been described that is not homologous in amino acid sequence or kinetic properties to the well-studied PPases (types A and B) found in many organisms from bacteria to humans and thought to be essential to the cell. Structural studies of the type-C PPases from Streptococcus gordonii and Bacillus subtilis reveal a homodimeric structure, with each polypeptide folding into two domains joined by a flexible hinge. The active site, formed at the interface between the N and C-terminal domains, binds two manganese ions approximately 3.6 A apart in a conformation resembling binuclear metal centres found in other hydrolytic enzymes. An activated water molecule bridging the two metal ions is likely poised for nucleophilic attack of the substrate. Importantly, the S. gordonii and B. subtilis enzymes have crystallised in strikingly different conformations. In both subunits of the S. gordonii crystal structure (1.5 A resolution) the C-terminal domain is positioned such that the active site is occluded, with a sulphate ion bound in the active site. In contrast, in the B. subtilis structure (3.0 A resolution) the C-terminal domain is rotated by about 90 degrees, leaving the active site wide open and accessible for substrate binding.
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{{ABSTRACT_PUBMED_11697905}}


==About this Structure==
==About this Structure==
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[[Category: Inorganic pyrophosphatase]]
[[Category: Inorganic pyrophosphatase]]
[[Category: Manganese]]
[[Category: Manganese]]
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Revision as of 18:21, 1 July 2008

File:1k23.png

Template:STRUCTURE 1k23

Inorganic Pyrophosphatase (Family II) from Bacillus subtilis

Template:ABSTRACT PUBMED 11697905

About this Structure

1K23 is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.

Reference

The "open" and "closed" structures of the type-C inorganic pyrophosphatases from Bacillus subtilis and Streptococcus gordonii., Ahn S, Milner AJ, Futterer K, Konopka M, Ilias M, Young TW, White SA, J Mol Biol. 2001 Nov 2;313(4):797-811. PMID:11697905

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