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| {{STRUCTURE_1k2g| PDB=1k2g | SCENE= }} | | {{STRUCTURE_1k2g| PDB=1k2g | SCENE= }} |
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| '''Structural basis for the 3'-terminal guanosine recognition by the group I intron'''
| | ===Structural basis for the 3'-terminal guanosine recognition by the group I intron=== |
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| ==Overview==
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| In the second step of the two consecutive transesterifications of the self-splicing reaction of the group I intron, the conserved guanosine at the 3' terminus of the intron (omegaG) binds to the guanosine-binding site (GBS) in the intron. In the present study, we designed a 22-nt model RNA (GBS/omegaG) including the GBS and omegaG from the Tetrahymena group I intron, and determined the solution structure by NMR methods. In this structure, omegaG is recognized by the formation of a base triple with the G264 x C311 base pair, and this recognition is stabilized by the stacking interaction between omegaG and C262. The bulged structure at A263 causes a large helical twist angle (40 +/- 80) between the G264 x C311 and C262 x G312 base pairs. We named this type of binding pocket with a bulge and a large twist, formed on the major groove, a "Bulge-and-Twist" (BT) pocket. With another twist angle between the C262 x G312 and G413 x C313 base pairs (45 +/- 100), the axis of GBS/omegaG is kinked at the GBS region. This kinked axis superimposes well on that of the corresponding region in the structure model built on a 5.0 A resolution electron density map (Golden et al., Science, 1998, 282:345-358). This compact structure of the GBS is also consistent with previous biochemical studies on group I introns. The BT pockets are also found in the arginine-binding site of the HIV-TAR RNA, and within the 16S rRNA and the 23S rRNA.
| | The line below this paragraph, {{ABSTRACT_PUBMED_11991639}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 11991639 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_11991639}} |
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| ==About this Structure== | | ==About this Structure== |
| Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K2G OCA]. | | Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K2G OCA]. |
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| ==Reference== | | ==Reference== |
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| [[Category: Gaga tetraloop]] | | [[Category: Gaga tetraloop]] |
| [[Category: Uucg tetraloop]] | | [[Category: Uucg tetraloop]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:13:19 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 21:29:53 2008'' |
