1k6d: Difference between revisions

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[[Image:1k6d.~~gif~~|left|200px]]

[[Image:1k6d.png|left|200px]]

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~~'''~~CRYSTAL STRUCTURE OF ACETATE COA-TRANSFERASE ALPHA SUBUNIT~~'''~~

===CRYSTAL STRUCTURE OF ACETATE COA-TRANSFERASE ALPHA SUBUNIT===

~~==Overview==~~

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The automation of protein structure determination is an essential component for high-throughput structural analysis in protein X-ray crystallography and is a key element in structural genomics. This highly challenging undertaking relies at present on the ~~availability of high-quality native and derivatized protein crystals diffracting~~ to ~~high or moderate resolution, respectively~~. ~~Obtaining such crystals often requires significant effort~~. ~~The present study demonstrates that phases obtained at low resolution (>3.0 A~~) ~~from crystals of SeMet-labeled protein can be successfully used for automated structure determination~~. ~~The crystal structure of acetate CoA~~-~~transferase alpha~~-~~subunit was solved using 3.4 A multi-wavelength anomalous dispersion data collected from a crystal containing SeMet-substituted protein and 1.9 A data collected from a native protein crystal.~~

The line below this paragraph, {{ABSTRACT_PUBMED_12454473}}, adds the Publication Abstract to the page

(as it appears on PubMed at http://www.pubmed.gov), where 12454473 is the PubMed ID number.

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==About this Structure==

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[[Category: Psi]]

[[Category: Structural genomic]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Fri May~~ 2 22:21:37 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 09:50:56 2008''

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-[[Image:1k6d.gif|left|200px]]
+{{Seed}}
+[[Image:1k6d.png|left|200px]]
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 {{STRUCTURE_1k6d|  PDB=1k6d  |  SCENE=  }}
-'''CRYSTAL STRUCTURE OF ACETATE COA-TRANSFERASE ALPHA SUBUNIT'''
+===CRYSTAL STRUCTURE OF ACETATE COA-TRANSFERASE ALPHA SUBUNIT===
-==Overview==
+<!--
-The automation of protein structure determination is an essential component for high-throughput structural analysis in protein X-ray crystallography and is a key element in structural genomics. This highly challenging undertaking relies at present on the availability of high-quality native and derivatized protein crystals diffracting to high or moderate resolution, respectively. Obtaining such crystals often requires significant effort. The present study demonstrates that phases obtained at low resolution (&gt;3.0 A) from crystals of SeMet-labeled protein can be successfully used for automated structure determination. The crystal structure of acetate CoA-transferase alpha-subunit was solved using 3.4 A multi-wavelength anomalous dispersion data collected from a crystal containing SeMet-substituted protein and 1.9 A data collected from a native protein crystal.
+The line below this paragraph, {{ABSTRACT_PUBMED_12454473}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 12454473 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_12454473}}
 ==About this Structure==
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 [[Category: Psi]]
 [[Category: Structural genomic]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 22:21:37 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul  2 09:50:56 2008''