8g0p: Difference between revisions

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'''Unreleased structure'''


The entry 8g0p is ON HOLD
==Crystal structure of the human Ndc80:Nuf2 loop region==
<StructureSection load='8g0p' size='340' side='right'caption='[[8g0p]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8g0p]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8G0P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8G0P FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8g0p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8g0p OCA], [https://pdbe.org/8g0p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8g0p RCSB], [https://www.ebi.ac.uk/pdbsum/8g0p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8g0p ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The conserved Ndc80 kinetochore complex, Ndc80c, is the principal link between mitotic spindle microtubules and centromere-associated proteins. We used AlphaFold 2 (AF2) to obtain predictions of the Ndc80 'loop' structure and of the Ndc80 : Nuf2 globular head domains that interact with the Dam1 subunit of the heterodecameric DASH/Dam1 complex (Dam1c). The predictions guided design of crystallizable constructs, with structures close to the predicted ones. The Ndc80 'loop' is a stiff, alpha-helical 'switchback' structure; AF2 predictions and positions of preferential cleavage sites indicate that flexibility within the long Ndc80c rod occurs instead at a hinge closer to the globular head. Conserved stretches of the Dam1 C terminus bind Ndc80c such that phosphorylation of Dam1 serine residues 257, 265 and 292 by the mitotic kinase Ipl1/Aurora B can release this contact during error correction of mis-attached kinetochores. We integrate the structural results presented here into our current molecular model of the kinetochore-microtubule interface. The model illustrates how multiple interactions between Ndc80c, DASH/Dam1c and the microtubule lattice stabilize kinetochore attachments.


Authors: Zahm, J.A., Jenni, S., Harrison, S.C.
Structure of the Ndc80 complex and its interactions at the yeast kinetochore-microtubule interface.,Zahm JA, Jenni S, Harrison SC Open Biol. 2023 Mar;13(3):220378. doi: 10.1098/rsob.220378. Epub 2023 Mar 8. PMID:36883282<ref>PMID:36883282</ref>


Description: Crystal structure of the human Ndc80:Nuf2 loop region
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Harrison, S.C]]
<div class="pdbe-citations 8g0p" style="background-color:#fffaf0;"></div>
[[Category: Jenni, S]]
== References ==
[[Category: Zahm, J.A]]
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Harrison SC]]
[[Category: Jenni S]]
[[Category: Zahm JA]]
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