BASIL2023GV1ZBS: Difference between revisions
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== '''Introduction''' == | == '''Introduction''' == | ||
<StructureSection load='1zbs' size='340' side='right' caption='1ZBS dimer as in its original state' scene=''> | <StructureSection load='1zbs' size='340' side='right' caption='1ZBS dimer as in its original state' scene=''> | ||
1ZBS is a protein with unknown function discovered through a genomics project of predictive folding. The goal of this project was to determine what the function of protein 1ZBS was using a combination of computational analysis and physical lab work. [[Image: FlowChart.png ]] | 1ZBS is a protein with unknown function discovered through a genomics project of predictive folding. The goal of this project was to determine what the function of protein 1ZBS was using a combination of computational analysis and physical lab work. | ||
[[Image: FlowChart.png ]] | |||
For this project, we started by doing our computational work, while we started transforming our bacteria, and over-expressing and purifying our protein for the kinase assays. | For this project, we started by doing our computational work, while we started transforming our bacteria, and over-expressing and purifying our protein for the kinase assays. | ||
== '''Sequence Alignments''' == | == '''Sequence Alignments''' == | ||
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=== BLAST-P === | === BLAST-P === | ||
The first type of analysis that was done was using Protein Blast from NCBI. The analysis was first done as it only gave very broad information about the potential function of the protein, by matching domains across other proteins with known functions. [[Image:BLASTPFigure.png]] | The first type of analysis that was done was using Protein Blast from NCBI. The analysis was first done as it only gave very broad information about the potential function of the protein, by matching domains across other proteins with known functions. | ||
[[Image:BLASTPFigure.png]] | |||
== '''Structural Alignments''' == | == '''Structural Alignments''' == | ||
