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[[Image:1kbm.gif|left|200px]]
{{Seed}}
[[Image:1kbm.png|left|200px]]


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{{STRUCTURE_1kbm|  PDB=1kbm  |  SCENE=  }}  
{{STRUCTURE_1kbm|  PDB=1kbm  |  SCENE=  }}  


'''SOLUTION STRUCTURE OF AN 11-MER DNA DUPLEX CONTAINING 6-THIOGUANINE OPPOSITE THYMINE'''
===SOLUTION STRUCTURE OF AN 11-MER DNA DUPLEX CONTAINING 6-THIOGUANINE OPPOSITE THYMINE===




==Overview==
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The incorporation of 6-thioguanine (S6G) into DNA is an essential step in the cytotoxic activity of thiopurines. However, the structural effects of this substitution on duplex DNA have not been fully characterized. Here, we present the solution structures of DNA duplexes containing S6G opposite thymine (S6G.T) and opposite cytosine (S6G.C), solved by high-resolution NMR spectroscopy and restrained molecular dynamics. The data indicate that both duplexes adopt right-handed helical conformations with all Watson-Crick hydrogen bonding in place. The S6G.T structures exhibit a wobble-type base pairing at the lesion site, with thymine shifted toward the major groove and S6G displaced toward the minor groove. Aside from the lesion site, the helices, including the flanking base pairs, are not highly perturbed by the presence of the lesion. Surprisingly, thermal dependence experiments suggest greater stability in the S6G-T mismatch than the S6G-C base pair.
The line below this paragraph, {{ABSTRACT_PUBMED_12582253}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 12582253 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12582253}}


==About this Structure==
==About this Structure==
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KBM OCA].  
Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KBM OCA].  


==Reference==
==Reference==
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[[Category: Thioguanine]]
[[Category: Thioguanine]]
[[Category: Thiopurine]]
[[Category: Thiopurine]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:32:49 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 10:05:29 2008''

Revision as of 07:05, 2 July 2008

File:1kbm.png

Template:STRUCTURE 1kbm

SOLUTION STRUCTURE OF AN 11-MER DNA DUPLEX CONTAINING 6-THIOGUANINE OPPOSITE THYMINE

Template:ABSTRACT PUBMED 12582253

About this Structure

Full experimental information is available from OCA.

Reference

Structural effect of the anticancer agent 6-thioguanine on duplex DNA., Bohon J, de los Santos CR, Nucleic Acids Res. 2003 Feb 15;31(4):1331-8. PMID:12582253

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