1jfi: Difference between revisions
New page: left|200px<br /> <applet load="1jfi" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jfi, resolution 2.62Å" /> '''Crystal Structure o... |
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[[Image:1jfi.gif|left|200px]]<br /> | [[Image:1jfi.gif|left|200px]]<br /><applet load="1jfi" size="350" color="white" frame="true" align="right" spinBox="true" | ||
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caption="1jfi, resolution 2.62Å" /> | caption="1jfi, resolution 2.62Å" /> | ||
'''Crystal Structure of the NC2-TBP-DNA Ternary Complex'''<br /> | '''Crystal Structure of the NC2-TBP-DNA Ternary Complex'''<br /> | ||
==Overview== | ==Overview== | ||
The X-ray structure of a ternary complex of Negative Cofactor 2 (NC2), the | The X-ray structure of a ternary complex of Negative Cofactor 2 (NC2), the TATA box binding protein (TBP), and DNA has been determined at 2.6 A resolution. The N termini of NC2 alpha and beta resemble histones H2A and H2B, respectively, and form a heterodimer that binds to the bent DNA double helix on the underside of the preformed TBP-DNA complex via electrostatic interactions. NC2beta contributes to inhibition of TATA-dependent transcription through interactions of its C-terminal alpha helix with a conserved hydrophobic feature on the upper surface of TBP, which in turn positions the penultimate alpha helix of NC2beta to block recognition of the TBP-DNA complex by transcription factor IIB. Further regulatory implications of the NC2 heterodimer structure are discussed. | ||
==Disease== | ==Disease== | ||
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==About this Structure== | ==About this Structure== | ||
1JFI is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. The following page contains interesting information on the relation of 1JFI with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb67_1.html TATA-Binding Protein]]. Full crystallographic information is available from [http:// | 1JFI is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. The following page contains interesting information on the relation of 1JFI with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb67_1.html TATA-Binding Protein]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JFI OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: TATA-Binding Protein]] | [[Category: TATA-Binding Protein]] | ||
[[Category: Burley, S | [[Category: Burley, S K.]] | ||
[[Category: Chen, H.]] | [[Category: Chen, H.]] | ||
[[Category: Kamada, K.]] | [[Category: Kamada, K.]] | ||
[[Category: Malik, S.]] | [[Category: Malik, S.]] | ||
[[Category: Meisterernst, M.]] | [[Category: Meisterernst, M.]] | ||
[[Category: NYSGXRC, New | [[Category: NYSGXRC, New York Structural GenomiX Research Consortium.]] | ||
[[Category: Roeder, R | [[Category: Roeder, R G.]] | ||
[[Category: Shu, F.]] | [[Category: Shu, F.]] | ||
[[Category: Stelzer, G.]] | [[Category: Stelzer, G.]] | ||
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[[Category: transcription initiation]] | [[Category: transcription initiation]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:22:04 2008'' | ||
Revision as of 11:22, 21 February 2008
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Crystal Structure of the NC2-TBP-DNA Ternary Complex
Overview
The X-ray structure of a ternary complex of Negative Cofactor 2 (NC2), the TATA box binding protein (TBP), and DNA has been determined at 2.6 A resolution. The N termini of NC2 alpha and beta resemble histones H2A and H2B, respectively, and form a heterodimer that binds to the bent DNA double helix on the underside of the preformed TBP-DNA complex via electrostatic interactions. NC2beta contributes to inhibition of TATA-dependent transcription through interactions of its C-terminal alpha helix with a conserved hydrophobic feature on the upper surface of TBP, which in turn positions the penultimate alpha helix of NC2beta to block recognition of the TBP-DNA complex by transcription factor IIB. Further regulatory implications of the NC2 heterodimer structure are discussed.
Disease
Known diseases associated with this structure: Glioblastoma OMIM:[606417], Huntington disease-like-4 OMIM:[600075], Parkinson disease OMIM:[600075], Spinocerebellar ataxia 17 OMIM:[600075]
About this Structure
1JFI is a Protein complex structure of sequences from Homo sapiens. The following page contains interesting information on the relation of 1JFI with [TATA-Binding Protein]. Full crystallographic information is available from OCA.
Reference
Crystal structure of negative cofactor 2 recognizing the TBP-DNA transcription complex., Kamada K, Shu F, Chen H, Malik S, Stelzer G, Roeder RG, Meisterernst M, Burley SK, Cell. 2001 Jul 13;106(1):71-81. PMID:11461703
Page seeded by OCA on Thu Feb 21 13:22:04 2008
Proteopedia Page Contributors and Editors (what is this?)
- Pages with broken file links
- Homo sapiens
- Protein complex
- TATA-Binding Protein
- Burley, S K.
- Chen, H.
- Kamada, K.
- Malik, S.
- Meisterernst, M.
- NYSGXRC, New York Structural GenomiX Research Consortium.
- Roeder, R G.
- Shu, F.
- Stelzer, G.
- H2a/h2b
- Histone
- Nc2
- Negative cofactor
- New york structural genomix research consortium
- Nysgxrc
- Protein structure initiative
- Psi
- Structural genomics
- Tata-dna
- Tbp
- Transcription initiation
