1kd4: Difference between revisions

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[[Image:1kd4.gif|left|200px]]
{{Seed}}
[[Image:1kd4.png|left|200px]]


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{{STRUCTURE_1kd4|  PDB=1kd4  |  SCENE=  }}  
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'''The Crystal Structure of r(GGUCACAGCCC)2, Barium form'''
===The Crystal Structure of r(GGUCACAGCCC)2, Barium form===




==Overview==
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Crystals of small RNAs, which regularly diffract to very high resolution, can often be readily obtained. Unfortunately, for some RNAs the conformations adopted in the crystalline form are different from those found in solution. For example, short RNAs that form hairpins in solution virtually never crystallize thus; rather, they form duplexes. Nevertheless, these unintended structures have contributed greatly to the understanding of RNA structure. In a similar occurrence, the homodimer r(GGUCACAGCCC)(2) has been crystallized from an 11-mer/12-mer heteroduplex, r(GGCUGAAGUCCG)/r(GGUCACAGCCC). This surprising phenomenon was observed under a variety of crystallization conditions. The structure of the homoduplex was determined from crystals that differed in the precipitant used and the type of metal present. In all cases, the resulting homoduplexes contain ten base pairings, of which the central six are non-canonical pairings. In two of the variants, ordered metal-binding sites were observed: two equivalent octacoordinate Tl(+) sites in one and two equivalent nanocoordinate Ba(2+) sites in another.
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{{ABSTRACT_PUBMED_12595698}}


==About this Structure==
==About this Structure==
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[[Category: Metal binding]]
[[Category: Metal binding]]
[[Category: Rna duplex]]
[[Category: Rna duplex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 10:09:19 2008''

Revision as of 07:09, 2 July 2008

File:1kd4.png

Template:STRUCTURE 1kd4

The Crystal Structure of r(GGUCACAGCCC)2, Barium form

Template:ABSTRACT PUBMED 12595698

About this Structure

Full crystallographic information is available from OCA.

Reference

Crystal structures of r(GGUCACAGCCC)2., Kacer V, Scaringe SA, Scarsdale JN, Rife JP, Acta Crystallogr D Biol Crystallogr. 2003 Mar;59(Pt 3):423-32. Epub 2003, Feb 21. PMID:12595698

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