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| {{STRUCTURE_1kdh| PDB=1kdh | SCENE= }} | | {{STRUCTURE_1kdh| PDB=1kdh | SCENE= }} |
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| '''Binary Complex of Murine Terminal Deoxynucleotidyl Transferase with a Primer Single Stranded DNA'''
| | ===Binary Complex of Murine Terminal Deoxynucleotidyl Transferase with a Primer Single Stranded DNA=== |
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| ==Overview==
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| The crystal structure of the catalytic core of murine terminal deoxynucleotidyltransferase (TdT) at 2.35 A resolution reveals a typical DNA polymerase beta-like fold locked in a closed form. In addition, the structures of two different binary complexes, one with an oligonucleotide primer and the other with an incoming ddATP-Co(2+) complex, show that the substrates and the two divalent ions in the catalytic site are positioned in TdT in a manner similar to that described for the human DNA polymerase beta ternary complex, suggesting a common two metal ions mechanism of nucleotidyl transfer in these two proteins. The inability of TdT to accommodate a template strand can be explained by steric hindrance at the catalytic site caused by a long lariat-like loop, which is absent in DNA polymerase beta. However, displacement of this discriminating loop would be sufficient to unmask a number of evolutionarily conserved residues, which could then interact with a template DNA strand. The present structure can be used to model the recently discovered human polymerase mu, with which it shares 43% sequence identity. | | The line below this paragraph, {{ABSTRACT_PUBMED_11823435}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 11823435 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_11823435}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Nucleotidyl transferase]] | | [[Category: Nucleotidyl transferase]] |
| [[Category: Polymerase]] | | [[Category: Polymerase]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:36:23 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 10:10:08 2008'' |
