1kdl: Difference between revisions

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[[Image:1kdl.gif|left|200px]]
{{Seed}}
[[Image:1kdl.png|left|200px]]


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{{STRUCTURE_1kdl|  PDB=1kdl  |  SCENE=  }}  
{{STRUCTURE_1kdl|  PDB=1kdl  |  SCENE=  }}  


'''Solution structure of the amphipathic domain of YopD from Yersinia'''
===Solution structure of the amphipathic domain of YopD from Yersinia===




==Overview==
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To establish an infection, Yersinia pseudotuberculosis utilizes a plasmid-encoded type III secretion machine that permits the translocation of several anti-host factors into the cytosol of target eukaryotic cells. Secreted YopD is essential for this process. Pre-secretory stabilization of YopD is mediated by an interaction with its cognate chaperone, LcrH. YopD possesses LcrH binding domains located in the N-terminus and in a predicted amphipathic domain located near the C-terminus. This latter domain is also critical for Yersinia virulence. In this study, we designed synthetic peptides encompassing the C-terminal amphipathic domain of YopD. A solution structure of YopD278-300, a peptide that strongly interacted with LcrH, was obtained by NMR methods. The structure is composed of a well-defined amphipathic alpha helix ranging from Phe280 to Tyr291, followed by a type I beta turn between residues Val292 and His295. The C-terminal truncated peptides, YopD278-292 and YopD271-292, lacked helical structure, implicating the beta turn in helix stability. An interaction between YopD278-300 and its cognate chaperone, LcrH, was observed by NMR through line-broadening effects and chemical shift differences between the free peptide and the peptide-LcrH complex. These effects were not observed for the unstructured peptide, YopD278-292, which confirms that the alpha helical structure of the YopD amphipathic domain is a critical binding region of LcrH.
The line below this paragraph, {{ABSTRACT_PUBMED_12153562}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 12153562 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12153562}}


==About this Structure==
==About this Structure==
1KDL is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KDL OCA].  
1KDL is a [[Single protein]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KDL OCA].  


==Reference==
==Reference==
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[[Category: Yersinia]]
[[Category: Yersinia]]
[[Category: Yopd]]
[[Category: Yopd]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:36:41 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 10:10:37 2008''

Revision as of 07:10, 2 July 2008

File:1kdl.png

Template:STRUCTURE 1kdl

Solution structure of the amphipathic domain of YopD from Yersinia

Template:ABSTRACT PUBMED 12153562

About this Structure

1KDL is a Single protein structure. Full experimental information is available from OCA.

Reference

Conformational analysis by CD and NMR spectroscopy of a peptide encompassing the amphipathic domain of YopD from Yersinia., Tengel T, Sethson I, Francis MS, Eur J Biochem. 2002 Aug;269(15):3659-68. PMID:12153562

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