Sandbox Reserved 1777: Difference between revisions

MRASA binds to SHOC2 exclusively through this concave region <Ref name='Kwan'>Kwon, J.J., Hajian, B., Bian, Y. et al. Structure–function analysis of the SHOC2–MRAS–PP1C holophosphatase complex. Nature 609, 408–415 (2022).doi: 10.1038/s41586-022-04928-2. [https://doi.org/10.1038/s41586-022-04928-2. DOI:10.1038/s41586-022-04928-2] </Ref>, primarily by the descending loop and strands of each LRR domains 2-10. This reaction is stabilized through <scene name='95/952705/Mras_structure/3'>hydrogen bonding</scene>.  PP1C <scene name='95/952706/Shoc2_pp1c_interaction/2'> associates </scene> with the ascending loops of the SHOC2 LRR regions, and is further engaged through the N-terminal region containing the <scene name='95/952705/Shoc2_rvxf/1'>RVxF</scene> motif <Ref name= 'Jajian'>Kwon, J., Jajian, B., Bian, Y. et al. Comprehensive structure-function evaluation of the SHOC2 holophosphatase reveals disease mechanisms and therapeutic opportunities. In: Proceedings of the American Association for Cancer Research Annual Meeting 2022. [https://aacrjournals.org/cancerres/article/82/12_Supplement/LB029/699443. DOI: 10.1158/1538-7445.AM2022-LB029]. </Ref>. The initial forming of the complex begins with SHOC2:PP1C engagement, then is completed and stabilized by the GTP-loaded MRAS binding <Ref name="Jajian" />. Once MRAS has associated with SHOC2, it <scene name='95/952705/Mras_pp1c_interaction/1'>binds</scene> to PP1C and guides the holoenzyme complex to the cell membrane to begin signaling<ref name="Kwan" />.