Sandbox Reserved 1769: Difference between revisions

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== Structure ==

NTCP is a transmembrane protein found in hepatocyte cells. It consists of nine <scene name='95/952697/Ntcp_open-pore_state/22'>transmembrane alpha helices</scene>, with the N-terminus located on the extracellular side of the plasma membrane and the C-terminus located on the intracellular side (Figure 3). ~~Transmembrane~~ helices are connected by short loops as well as extracellular and intracellular alpha helices that lie nearly parallel to the membrane.<ref name="Asami" /> Structures were determined by [https://en.wikipedia.org/wiki/Cryogenic_electron_microscopy cryogenic electron microscopy (Cryo-EM)] of NTCP in complex with antibodies or nanobodies. <ref name="Goutam" /> <ref name="Asami" /> <Ref name="Park"> Park JH, Iwamoto M, Yun JH, Uchikubo-Kamo T, Son D, Jin Z, Yoshida H, Ohki M, Ishimoto N, Mizutani K, Oshima M, Muramatsu M, Wakita T, Shirouzu M, Liu K, Uemura T, Nomura N, Iwata S, Watashi K, Tame JRH, Nishizawa T, Lee W, Park SY. Structural insights into the HBV receptor and bile acid transporter NTCP. Nature. 2022 Jun;606(7916):1027-1031. [https://dx.doi.org/10.1038/s41586-022-04857-0 DOI: 10.1038/s41586-022-04857-0]. </Ref> <ref name="Liu" />

NTCP is a transmembrane protein found in hepatocyte cells. It consists of nine <scene name='95/952697/Ntcp_open-pore_state/22'>transmembrane alpha helices</scene>, with the N-terminus located on the extracellular side of the plasma membrane and the C-terminus located on the intracellular side (Figure 3). The transmembrane helices are connected by short loops as well as extracellular and intracellular alpha helices that lie nearly parallel to the membrane.<ref name="Asami" /> Structures of NTCP were determined by [https://en.wikipedia.org/wiki/Cryogenic_electron_microscopy cryogenic electron microscopy (Cryo-EM)] of NTCP in complex with antibodies or nanobodies. <ref name="Goutam" /> <ref name="Asami" /> <Ref name="Park"> Park JH, Iwamoto M, Yun JH, Uchikubo-Kamo T, Son D, Jin Z, Yoshida H, Ohki M, Ishimoto N, Mizutani K, Oshima M, Muramatsu M, Wakita T, Shirouzu M, Liu K, Uemura T, Nomura N, Iwata S, Watashi K, Tame JRH, Nishizawa T, Lee W, Park SY. Structural insights into the HBV receptor and bile acid transporter NTCP. Nature. 2022 Jun;606(7916):1027-1031. [https://dx.doi.org/10.1038/s41586-022-04857-0 DOI: 10.1038/s41586-022-04857-0]. </Ref> <ref name="Liu" />

[[Image:Topology_picture.png|300 px|thumb|Figure 3. NTCP topology.]]

[[Image:Topology_picture.png|300 px|thumb|Figure 3. NTCP topology. Transmembrane helices are numbered. TM helices comprising the panel domain are shown in orange. TM helices comprising the core domain are shown in shades of blue. Light and dark blue shades represent different helix bundles of the core domain, which together demonstrate pseudo two-fold symmetry.]]

=== Domains ===

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@@ Line 7: / Line 7: @@
 == Structure ==
-NTCP is a transmembrane protein found in hepatocyte cells. It consists of nine <scene name='95/952697/Ntcp_open-pore_state/22'>transmembrane alpha helices</scene>, with the N-terminus located on the extracellular side of the plasma membrane and the C-terminus located on the intracellular side (Figure 3). Transmembrane helices are connected by short loops as well as extracellular and intracellular alpha helices that lie nearly parallel to the membrane.<ref name="Asami" /> Structures were determined by [https://en.wikipedia.org/wiki/Cryogenic_electron_microscopy cryogenic electron microscopy (Cryo-EM)] of NTCP in complex with antibodies or nanobodies. <ref name="Goutam" /> <ref name="Asami" /> <Ref name="Park"> Park JH, Iwamoto M, Yun JH, Uchikubo-Kamo T, Son D, Jin Z, Yoshida H, Ohki M, Ishimoto N, Mizutani K, Oshima M, Muramatsu M, Wakita T, Shirouzu M, Liu K, Uemura T, Nomura N, Iwata S, Watashi K, Tame JRH, Nishizawa T, Lee W, Park SY. Structural insights into the HBV receptor and bile acid transporter NTCP. Nature. 2022 Jun;606(7916):1027-1031. [https://dx.doi.org/10.1038/s41586-022-04857-0 DOI: 10.1038/s41586-022-04857-0]. </Ref> <ref name="Liu" />
+NTCP is a transmembrane protein found in hepatocyte cells. It consists of nine <scene name='95/952697/Ntcp_open-pore_state/22'>transmembrane alpha helices</scene>, with the N-terminus located on the extracellular side of the plasma membrane and the C-terminus located on the intracellular side (Figure 3). The transmembrane helices are connected by short loops as well as extracellular and intracellular alpha helices that lie nearly parallel to the membrane.<ref name="Asami" /> Structures of NTCP were determined by [https://en.wikipedia.org/wiki/Cryogenic_electron_microscopy cryogenic electron microscopy (Cryo-EM)] of NTCP in complex with antibodies or nanobodies. <ref name="Goutam" /> <ref name="Asami" /> <Ref name="Park"> Park JH, Iwamoto M, Yun JH, Uchikubo-Kamo T, Son D, Jin Z, Yoshida H, Ohki M, Ishimoto N, Mizutani K, Oshima M, Muramatsu M, Wakita T, Shirouzu M, Liu K, Uemura T, Nomura N, Iwata S, Watashi K, Tame JRH, Nishizawa T, Lee W, Park SY. Structural insights into the HBV receptor and bile acid transporter NTCP. Nature. 2022 Jun;606(7916):1027-1031. [https://dx.doi.org/10.1038/s41586-022-04857-0 DOI: 10.1038/s41586-022-04857-0]. </Ref> <ref name="Liu" />
-[[Image:Topology_picture.png|300 px|thumb|Figure 3. NTCP topology.]]
+[[Image:Topology_picture.png|300 px|thumb|Figure 3. NTCP topology. Transmembrane helices are numbered. TM helices comprising the panel domain are shown in orange. TM helices comprising the core domain are shown in shades of blue. Light and dark blue shades represent different helix bundles of the core domain, which together demonstrate pseudo two-fold symmetry.]]
 === Domains ===