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| {{STRUCTURE_1kfa| PDB=1kfa | SCENE= }} | | {{STRUCTURE_1kfa| PDB=1kfa | SCENE= }} |
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| '''Crystal structure of Fab fragment complexed with gibberellin A4'''
| | ===Crystal structure of Fab fragment complexed with gibberellin A4=== |
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| ==Overview==
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| Gibberellins, a class of plant hormones, consist of more than 120 members. Only a few of them are recognized by a receptor that remains unknown. The haptenic mouse monoclonal antibody, 4-B8(8)/E9, was generated against gibberellin A(4) (GA(4)) to recognize biologically active GA selectivity, and we attempted to confirm the binding properties between the antibody and GA(4). We carried out an X-ray crystallographic analysis of the 4-B8(8)/E9 Fab fragment complexed with GA(4) at a 2.8 A resolution by using the molecular replacement method. The crystal structure of the Fab fragment showed the typical immunoglobulin fold of the beta-barrel structure which is the common motif of all antibodies. A small hapten-combining site was made up of three heavy chain CDR loops. On the other hand, CDRs of the light chain did not interact directly with GA(4). The C/D rings of the GA(4) molecule were in van der Waals contact mainly with the aromatic side chain of Tyr100AH and Phe100BH of CDR-H3. The 3 beta-hydroxyl and 6 beta-carboxyl groups were, respectively, hydrogen-bonded to the main chain of Ala33H and to the Thr53H heavy chain.
| | The line below this paragraph, {{ABSTRACT_PUBMED_12054627}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 12054627 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_12054627}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Yamaguchi, I.]] | | [[Category: Yamaguchi, I.]] |
| [[Category: Immunoglobuiln fold]] | | [[Category: Immunoglobuiln fold]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:40:30 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 10:15:21 2008'' |
