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| [[Image:1kfn.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_1kfn| PDB=1kfn | SCENE= }} | | {{STRUCTURE_1kfn| PDB=1kfn | SCENE= }} |
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| '''Core side-chain packing and backbone conformation in Lpp-56 coiled-coil mutants'''
| | ===Core side-chain packing and backbone conformation in Lpp-56 coiled-coil mutants=== |
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| ==Overview==
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| Native proteins exhibit precise geometric packing of atoms in their hydrophobic interiors. Nonetheless, controversy remains about the role of core side-chain packing in specifying and stabilizing the folded structures of proteins. Here we investigate the role of core packing in determining the conformation and stability of the Lpp-56 trimerization domain. The X-ray crystal structures of Lpp-56 mutants with alanine substitutions at two and four interior core positions reveal trimeric coiled coils in which the twist of individual helices and the helix-helix spacing vary significantly to achieve the most favored superhelical packing arrangement. Introduction of each alanine "layer" into the hydrophobic core destabilizes the superhelix by 1.4 kcal mol(-1). Although the methyl groups of the alanine residues pack at their optimum van der Waals contacts in the coiled-coil trimer, they provide a smaller component of hydrophobic interactions than bulky hydrophobic side-chains to the thermodynamic stability. Thus, specific side-chain packing in the hydrophobic core of coiled coils are important determinants of protein main-chain conformation and stability.
| | The line below this paragraph, {{ABSTRACT_PUBMED_12054830}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 12054830 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_12054830}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Lipoprotein]] | | [[Category: Lipoprotein]] |
| [[Category: Protein folding]] | | [[Category: Protein folding]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:41:22 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 10:16:25 2008'' |
