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[[Image:1kfo.gif|left|200px]]
{{Seed}}
[[Image:1kfo.png|left|200px]]


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{{STRUCTURE_1kfo|  PDB=1kfo  |  SCENE=  }}  
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'''CRYSTAL STRUCTURE OF AN RNA HELIX RECOGNIZED BY A ZINC-FINGER PROTEIN: AN 18 BASE PAIR DUPLEX AT 1.6 RESOLUTION'''
===CRYSTAL STRUCTURE OF AN RNA HELIX RECOGNIZED BY A ZINC-FINGER PROTEIN: AN 18 BASE PAIR DUPLEX AT 1.6 RESOLUTION===




==Overview==
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The crystal structure of the 19-mer RNA, 5'-GAAUGCCUGCGAGCAUCCC-3' has been determined from X-ray diffraction data to 1.6 A resolution by the multiwavelength anomalous diffraction method from crystals containing a brominated uridine. In the crystal, this RNA forms an 18-mer self-complementary double helix with the 19th nucleotide flipped out of the helix. This helix contains most of the target stem recognized by the bacteriophage Mu Com protein (control of mom), which activates translation of an unusual DNA modification enzyme, Mom. The 19-mer duplex, which contains one A.C mismatch and one A.C/G.U tandem wobble pair, was shown to bind to the Com protein by native gel electrophoresis shift assay. Comparison of the geometries and base stacking properties between Watson-Crick base pairs and the mismatches in the crystal structure suggest that both hydrogen bonding and base stacking are important for stabilizing these mismatched base pairs, and that the unusual geometry adopted by the A.C mismatch may reveal a unique structural motif required for the function of Com.
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{{ABSTRACT_PUBMED_12166647}}


==About this Structure==
==About this Structure==
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[[Category: Lima, S.]]
[[Category: Lima, S.]]
[[Category: Rna]]
[[Category: Rna]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:41:25 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 10:16:27 2008''

Revision as of 07:16, 2 July 2008

File:1kfo.png

Template:STRUCTURE 1kfo

CRYSTAL STRUCTURE OF AN RNA HELIX RECOGNIZED BY A ZINC-FINGER PROTEIN: AN 18 BASE PAIR DUPLEX AT 1.6 RESOLUTION

Template:ABSTRACT PUBMED 12166647

About this Structure

Full crystallographic information is available from OCA.

Reference

Crystal structure of an RNA helix recognized by a zinc-finger protein: an 18-bp duplex at 1.6 A resolution., Lima S, Hildenbrand J, Korostelev A, Hattman S, Li H, RNA. 2002 Jul;8(7):924-32. PMID:12166647

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