Sandbox Reserved 1790: Difference between revisions

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==SHOC2 and MRAS==

MRAS is initially bound to GDP causing it to be in its inactive state. This form cannot bind to the SHOC2-PP1C complex due to steric clashing. Once GDP is exchanged for GTP to activate the protein, <scene name='95/952716/~~conformational changes~~/1'>~~SHOC2-MRAS (residues)~~</scene> occur within the switch I and switch II regions to allow <scene name='95/952716/Scho2-mras-interactions/2'>MRAS to interact with SHOC2</scene>. These <scene name='95/952716/Scho2-mras-interactions/1'>interactions</scene> include hydrogen bonds and pi stacking. The primary hydrogen bonds are R288-Q71 and R177-E47. Pi staking occurs at R104-R83 <ref name="Lavoie">Lavoie H, Therrien M. Structural keys unlock RAS-MAPK cellular signalling pathway. Nature. 2022 Sep;609(7926):248-249. [http://dx.doi.org/10.1038/d41586-022-02189-7 doi: 10.1038/d41586-022-02189-7. PMID: 35970881.]</ref>.

MRAS is initially bound to GDP causing it to be in its inactive state. This form cannot bind to the SHOC2-PP1C complex due to steric clashing. Once GDP is exchanged for GTP to activate the protein, <scene name='95/952716/Scho2-mras-interactions/1'>conformational changes</scene> occur within the switch I and switch II regions to allow <scene name='95/952716/Scho2-mras-interactions/2'>MRAS to interact with SHOC2</scene>. These <scene name='95/952716/Scho2-mras-interactions/1'>interactions</scene> include hydrogen bonds and pi stacking. The primary hydrogen bonds are R288-Q71 and R177-E47. Pi staking occurs at R104-R83 <ref name="Lavoie">Lavoie H, Therrien M. Structural keys unlock RAS-MAPK cellular signalling pathway. Nature. 2022 Sep;609(7926):248-249. [http://dx.doi.org/10.1038/d41586-022-02189-7 doi: 10.1038/d41586-022-02189-7. PMID: 35970881.]</ref>.

==PP1C and MRAS==

Sandbox Reserved 1790: Difference between revisions

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@@ Line 25: / Line 25: @@
 ==SHOC2 and MRAS==
-MRAS is initially bound to GDP causing it to be in its inactive state. This form cannot bind to the SHOC2-PP1C complex due to steric clashing. Once GDP is exchanged for GTP to activate the protein, <scene name='95/952716/conformational changes/1'>SHOC2-MRAS (residues)</scene> occur within the switch I and switch II regions to allow <scene name='95/952716/Scho2-mras-interactions/2'>MRAS to interact with SHOC2</scene>. These <scene name='95/952716/Scho2-mras-interactions/1'>interactions</scene> include hydrogen bonds and pi stacking. The primary hydrogen bonds are R288-Q71 and R177-E47. Pi staking occurs at R104-R83 <ref name="Lavoie">Lavoie H, Therrien M. Structural keys unlock RAS-MAPK cellular signalling pathway. Nature. 2022 Sep;609(7926):248-249. [http://dx.doi.org/10.1038/d41586-022-02189-7 doi: 10.1038/d41586-022-02189-7. PMID: 35970881.]</ref>.
+MRAS is initially bound to GDP causing it to be in its inactive state. This form cannot bind to the SHOC2-PP1C complex due to steric clashing. Once GDP is exchanged for GTP to activate the protein, <scene name='95/952716/Scho2-mras-interactions/1'>conformational changes</scene> occur within the switch I and switch II regions to allow <scene name='95/952716/Scho2-mras-interactions/2'>MRAS to interact with SHOC2</scene>. These <scene name='95/952716/Scho2-mras-interactions/1'>interactions</scene> include hydrogen bonds and pi stacking. The primary hydrogen bonds are R288-Q71 and R177-E47. Pi staking occurs at R104-R83 <ref name="Lavoie">Lavoie H, Therrien M. Structural keys unlock RAS-MAPK cellular signalling pathway. Nature. 2022 Sep;609(7926):248-249. [http://dx.doi.org/10.1038/d41586-022-02189-7 doi: 10.1038/d41586-022-02189-7. PMID: 35970881.]</ref>.
 ==PP1C and MRAS==