BASIL2023GVQ8DN35: Difference between revisions
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[[Image:Docking site Q8DN35.png]] | [[Image:Docking site Q8DN35.png]] | ||
This image displays potential binding sites to the Q8DN35 protein. | This image displays potential binding sites to the Q8DN35 protein. Each grey "X" indicates a potential binding site. These binding sites were taken into consideration when looking at PyRx docking tools. | ||
== Experimental process == | == Experimental process == | ||
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== Q8DN35 DNA analysis == | == Q8DN35 DNA analysis == | ||
To confirm presence of Q8DN35 DNA, plasmid digestion was performed to observe correct DNA. | To confirm presence of Q8DN35 DNA, plasmid digestion was performed to observe correct DNA. Sizes were determined from looking at the plasmid map provided for our kinase enzyme DNA. Cutting with both of the chosen restriction enzymes yielded a smaller band containing our insert and a larger band containing the rest of the plasmid. Using only one restriction enzyme gives one band with all of the plasmid DNA present. | ||
[[Image:Q8DN35 DNA Gel.png]] | [[Image:Q8DN35 DNA Gel.png]] | ||
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== References == | == References == | ||
1.Miyazono, K., Tabei, N., Morita, S., Ohnishi, Y., Horinouchi, S., & Tanokura, M. (2012). Substrate recognition mechanism and substrate-dependent conformational changes of an ROK family glucokinase from Streptomyces griseus. Journal of bacteriology, 194(3), 607–616. https://doi.org/10.1128/JB.06173-11 | |||
2. The PyMOL Molecular Graphics System, Version 2.0 Schrödinger, LLC. | |||
<references/> | <references/> | ||
