Sandbox Reserved 1777: Difference between revisions
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This conformational change is caused by GTP replacing GDP. When GTP is bound, MRAS shifts and binds with the previously associated SHOC2-PP1C complex. When GDP is bound, switch II in MRAS is moved outward, which causes a steric clash with SHOC2 <scene name='95/952705/Switch_i_and_ii_with_gdp/8'>(Switch I and II with GDP)</scene>. When GTP is bound, switch II in MRAS can form various hydrogen bonding, pi stacking and hydrophobic interactions with SHOC2<Ref name='Bonsor'>Daniel A. Bonsor, Patrick Alexander, Kelly Snead, Nicole Hartig, Matthew Drew, Simon Messing, Lorenzo I. Finci, Dwight V. Nissley, Frank McCormick, Dominic Esposito, Pablo Rodrigiguez-Viciana, Andrew G. Stephen, Dhirendra K. Simanshu. Structure of the SHOC2–MRAS–PP1C complex provides insights into RAF activation and Noonan syndrome. bioRxiv. 2022.05.10.491335. doi: 10.1101/2022.05.10.491335. [https://doi.org/10.1101/2022.05.10.491335. DOI:10.1101/2022.05.10.491335]. </Ref> <scene name='95/952705/Switch_i_and_ii_with_gtp/5'>(Switch I and II with GTP)</scene>. When MRAS is bound to SHOC2-PP1C, switch I has an important role in making interactions with PP1C. | This conformational change is caused by GTP replacing GDP. When GTP is bound, MRAS shifts and binds with the previously associated SHOC2-PP1C complex. When GDP is bound, switch II in MRAS is moved outward, which causes a steric clash with SHOC2 <scene name='95/952705/Switch_i_and_ii_with_gdp/8'>(Switch I and II with GDP)</scene>. When GTP is bound, switch II in MRAS can form various hydrogen bonding, pi stacking and hydrophobic interactions with SHOC2<Ref name='Bonsor'>Daniel A. Bonsor, Patrick Alexander, Kelly Snead, Nicole Hartig, Matthew Drew, Simon Messing, Lorenzo I. Finci, Dwight V. Nissley, Frank McCormick, Dominic Esposito, Pablo Rodrigiguez-Viciana, Andrew G. Stephen, Dhirendra K. Simanshu. Structure of the SHOC2–MRAS–PP1C complex provides insights into RAF activation and Noonan syndrome. bioRxiv. 2022.05.10.491335. doi: 10.1101/2022.05.10.491335. [https://doi.org/10.1101/2022.05.10.491335. DOI:10.1101/2022.05.10.491335]. </Ref> <scene name='95/952705/Switch_i_and_ii_with_gtp/5'>(Switch I and II with GTP)</scene>. When MRAS is bound to SHOC2-PP1C, switch I has an important role in making interactions with PP1C. | ||
<scene name='95/954077/Gdp_with_mras_and_shoc2/2'>TextToBeDisplayed</scene> | |||
==Stabilizing Interactions in Ternary Complex== | ==Stabilizing Interactions in Ternary Complex== | ||
