8odw: Difference between revisions

Line 1:

~~'''Unreleased structure'''~~

~~The entry~~ 8odw is ~~ON HOLD until Paper~~ Publication

==Crystal structure of LbmA Ox-ACP didomain in complex with NADP and ethyl glycinate from the lobatamide PKS (Gynuella sunshinyii)==

== Structural highlights ==

<table><tr><td colspan='2'>[[8odw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Gynuella_sunshinyii Gynuella sunshinyii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8ODW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8ODW FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=GEE:ETHYL+GLYCINATE'>GEE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8odw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8odw OCA], [https://pdbe.org/8odw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8odw RCSB], [https://www.ebi.ac.uk/pdbsum/8odw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8odw ProSAT]</span></td></tr>

</table>

== Function ==

[https://www.uniprot.org/uniprot/A0A0C5VQJ2_9GAMM A0A0C5VQJ2_9GAMM]

== Publication Abstract from PubMed ==

Modular trans-acyltransferase polyketide synthases (trans-AT PKSs) are enzymatic assembly lines that biosynthesize complex polyketide natural products. Relative to their better studied cis-AT counterparts, the trans-AT PKSs introduce remarkable chemical diversity into their polyketide products. A notable example is the lobatamide A PKS, which incorporates a methylated oxime. Here we demonstrate biochemically that this functionality is installed on-line by an unusual oxygenase-containing bimodule. Furthermore, analysis of the oxygenase crystal structure coupled with site-directed mutagenesis allows us to propose a model for catalysis, as well as identifying key protein-protein interactions that support this chemistry. Overall, our work adds oxime-forming machinery to the biomolecular toolbox available for trans-AT PKS engineering, opening the way to introducing such masked aldehyde functionalities into diverse polyketides.

~~Authors:~~ Francois, ~~R.M.M.~~, Fraley, A~~.E.~~, Piel, J.~~, Weissman, K~~.J.~~, Gruez, A~~.

Modular oxime formation by a trans-AT polyketide synthase.,Minas HA, Francois RMM, Hemmerling F, Fraley AE, Dieterich CL, Rudisser SH, Meoded RA, Collin S, Weissman KJ, Gruez A, Piel J Angew Chem Int Ed Engl. 2023 May 22:e202304481. doi: 10.1002/anie.202304481. PMID:37216334<ref>PMID:37216334</ref>

~~Description: Crystal structure~~ of ~~LbmA Ox~~-~~ACP didomain in complex with NADP and ethyl glycinate from the lobatamide PKS (~~Gynuella sunshinyii)

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

[[Category: ~~Unreleased~~ Structures]]

</div>

[[Category: ~~Gruez, A~~]]

[[Category: ~~Fraley, A.E~~]]

== References ==

[[Category: ~~Weissman, K.J~~]]

[[Category: ~~Francois, R.M.M~~]]

__TOC__

[[Category: ~~Piel, J~~]]

</StructureSection>

[[Category: Gynuella sunshinyii]]

[[Category: Large Structures]]

[[Category: Fraley AE]]

[[Category: Francois RMM]]

[[Category: Gruez A]]

[[Category: Piel J]]

[[Category: Weissman KJ]]

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 8odw is ON HOLD  until Paper Publication
+==Crystal structure of LbmA Ox-ACP didomain in complex with NADP and ethyl glycinate from the lobatamide PKS (Gynuella sunshinyii)==
+<StructureSection load='8odw' size='340' side='right'caption='[[8odw]], [[Resolution|resolution]] 3.07&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[8odw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Gynuella_sunshinyii Gynuella sunshinyii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8ODW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8ODW FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=GEE:ETHYL+GLYCINATE'>GEE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8odw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8odw OCA], [https://pdbe.org/8odw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8odw RCSB], [https://www.ebi.ac.uk/pdbsum/8odw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8odw ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/A0A0C5VQJ2_9GAMM A0A0C5VQJ2_9GAMM]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Modular trans-acyltransferase polyketide synthases (trans-AT PKSs) are enzymatic assembly lines that biosynthesize complex polyketide natural products. Relative to their better studied cis-AT counterparts, the trans-AT PKSs introduce remarkable chemical diversity into their polyketide products. A notable example is the lobatamide A PKS, which incorporates a methylated oxime. Here we demonstrate biochemically that this functionality is installed on-line by an unusual oxygenase-containing bimodule. Furthermore, analysis of the oxygenase crystal structure coupled with site-directed mutagenesis allows us to propose a model for catalysis, as well as identifying key protein-protein interactions that support this chemistry. Overall, our work adds oxime-forming machinery to the biomolecular toolbox available for trans-AT PKS engineering, opening the way to introducing such masked aldehyde functionalities into diverse polyketides.
-Authors: Francois, R.M.M., Fraley, A.E., Piel, J., Weissman, K.J., Gruez, A.
+Modular oxime formation by a trans-AT polyketide synthase.,Minas HA, Francois RMM, Hemmerling F, Fraley AE, Dieterich CL, Rudisser SH, Meoded RA, Collin S, Weissman KJ, Gruez A, Piel J Angew Chem Int Ed Engl. 2023 May 22:e202304481. doi: 10.1002/anie.202304481. PMID:37216334<ref>PMID:37216334</ref>
-Description: Crystal structure of LbmA Ox-ACP didomain in complex with NADP and ethyl glycinate from the lobatamide PKS (Gynuella sunshinyii)
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Gruez, A]]
+<div class="pdbe-citations 8odw" style="background-color:#fffaf0;"></div>
-[[Category: Fraley, A.E]]
+== References ==
-[[Category: Weissman, K.J]]
+<references/>
-[[Category: Francois, R.M.M]]
+__TOC__
-[[Category: Piel, J]]
+</StructureSection>
+[[Category: Gynuella sunshinyii]]
+[[Category: Large Structures]]
+[[Category: Fraley AE]]
+[[Category: Francois RMM]]
+[[Category: Gruez A]]
+[[Category: Piel J]]
+[[Category: Weissman KJ]]