Sandbox Reserved 1774: Difference between revisions

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=== Importance of Hinge Region to Signaling Activation ===

[[Image:TSH Signaling.png|right|~~400~~ px|thumb|Figure 2: (Left) In the downright, inactive state, TSH cannot bind and no signaling activation occurs. (Right) In the upright, active state, binding of TSH leads to GPCR signaling activation and production of T3 and T4 hormones.]]

[[Image:TSH Signaling.png|right|500 px|thumb|Figure 2: (Left) In the downright, inactive state, TSH cannot bind and no signaling activation occurs. (Right) In the upright, active state, binding of TSH leads to GPCR signaling activation and production of T3 and T4 hormones.]]

The TSHR hinges between two states: <scene name='95/952702/Overlay/2'>active and inactive</scene>. When the extracellular domain is hinged down, the receptor is inactive and no signaling activation occurs. When the extracellular domain raises into the upright position, the hinge region deforms and interacts with the extracellular loops to cause a conformation change in the TM domain and corresponding G-protein activation <ref name="Bruser">Bruser A, Schulz A, Rothemund S, et al. The Activation Mechanism of Glycoprotein Hormone Receptors with Implications in the Cause and Therapy of Endocrine Diseases. J Biol Chem. 2016;291(2):508-520. [https://doi.org/10.1074/jbc.M115.701102 DOI:10.1074/jbc.M115.701102]</ref>. While transition between the active and inactive states occurs spontaneously, favoring of one state over the other is influence by hinge interactions and ligand binding <ref name="Faust">PMID:35940205</ref>. When stabilized in the upright conformation, the activated GPCR signaling pathway results in transcription of thyroid hormones T3 and T4 (Fig. __) <ref name="Chu" />.

Sandbox Reserved 1774: Difference between revisions

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@@ Line 18: / Line 18: @@
 === Importance of Hinge Region to Signaling Activation ===
-[[Image:TSH Signaling.png|right|400 px|thumb|Figure 2: (Left) In the downright, inactive state, TSH cannot bind and no signaling activation occurs. (Right) In the upright, active state, binding of TSH leads to GPCR signaling activation and production of T3 and T4 hormones.]]
+[[Image:TSH Signaling.png|right|500 px|thumb|Figure 2: (Left) In the downright, inactive state, TSH cannot bind and no signaling activation occurs. (Right) In the upright, active state, binding of TSH leads to GPCR signaling activation and production of T3 and T4 hormones.]]
 The TSHR hinges between two states: <scene name='95/952702/Overlay/2'>active and inactive</scene>. When the extracellular domain is hinged down, the receptor is inactive and no signaling activation occurs. When the extracellular domain raises into the upright position, the hinge region deforms and interacts with the extracellular loops to cause a conformation change in the TM domain and corresponding G-protein activation <ref name="Bruser">Bruser A, Schulz A, Rothemund S, et al. The Activation Mechanism of Glycoprotein Hormone Receptors with Implications in the Cause and Therapy of Endocrine Diseases. J Biol Chem. 2016;291(2):508-520. [https://doi.org/10.1074/jbc.M115.701102 DOI:10.1074/jbc.M115.701102]</ref>. While transition between the active and inactive states occurs spontaneously, favoring of one state over the other is influence by hinge interactions and ligand binding <ref name="Faust">PMID:35940205</ref>. When stabilized in the upright conformation, the activated GPCR signaling pathway results in transcription of thyroid hormones T3 and T4 (Fig. __) <ref name="Chu" />.