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| {{STRUCTURE_1klf| PDB=1klf | SCENE= }} | | {{STRUCTURE_1klf| PDB=1klf | SCENE= }} |
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| '''FIMH ADHESIN-FIMC CHAPERONE COMPLEX WITH D-MANNOSE'''
| | ===FIMH ADHESIN-FIMC CHAPERONE COMPLEX WITH D-MANNOSE=== |
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| ==Overview==
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| The first step in the colonization of the human urinary tract by pathogenic Escherichia coli is the mannose-sensitive binding of FimH, the adhesin present at the tip of type 1 pili, to the bladder epithelium. We elucidated crystallographically the interactions of FimH with D-mannose. The unique site binding pocket occupied by D-mannose was probed using site-directed mutagenesis. All but one of the mutants examined had greatly diminished mannose-binding activity and had also lost the ability to bind human bladder cells. The binding activity of the mono-saccharide D-mannose was delineated from this of mannotriose (Man(alpha 1-3)[Man(alpha 1-6)]Man) by generating mutants that abolished D-mannose binding but retained mannotriose binding activity. Our structure/function analysis demonstrated that the binding of the monosaccharide alpha-D-mannose is the primary bladder cell receptor for uropathogenic E. coli and that this event requires a highly conserved FimH binding pocket. The residues in the FimH mannose-binding pocket were sequenced and found to be invariant in over 200 uropathogenic strains of E. coli. Only enterohaemorrhagic E. coli (EHEC) possess a sequence variation within the mannose-binding pocket of FimH, suggesting a naturally occurring mechanism of attenuation in EHEC bacteria that would prevent them from being targeted to the urinary tract. | | The line below this paragraph, {{ABSTRACT_PUBMED_12010488}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 12010488 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_12010488}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Adhesin-chaperone complex]] | | [[Category: Adhesin-chaperone complex]] |
| [[Category: Mannose-bound]] | | [[Category: Mannose-bound]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:52:52 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 10:30:34 2008'' |
