BASIL2023GV1ZBS: Difference between revisions

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=== NetGO ===

We used NetGo 2.0 to help visualize the potential function of 1ZBS. This software uses predictive modeling to determine potential functions of the protein in query, and produces a set of (GO) terms that best aligns with the protein's possible function.

We used NetGo 2.0 to help visualize the potential function of 1ZBS. This software uses predictive modeling to determine potential functions of the protein in query, and produces a set of gene ontology(GO) terms that best aligns with the protein's possible function. It starts with the most general information and builds to become more specific. These results came back in a list form. We then used AmiGo to visualize the connection between the terms. The final results landed us with a protein that has a purine ribonucleotide triphosphate binding domain, and could have some NAG kinase activity.

==''' Docking Analysis'''==

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== '''Conclusion''' ==

The goal of this experiment was to determine the function of 1zbs. We did this by creating a standard curve of BSA to compare our curve to our possible substrate NAG. This was done by a series of dilutions and Bradford assays to obtain a dilution factor by comparing it the absorbance levels of BSA. We concluded from our kinase assay results that 1ZBS is not a kinase because the specific activity was 0.074 U/mg.

This is a sample scene created with SAT to <scene name="/12/3456/Sample/1">color</scene></scene> by Group, and another to make <scene name='95/957643/1zbs_view_2/1'> a transparent representation</scene> of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes.

</StructureSection>

== References ==

BASIL2023GV1ZBS: Difference between revisions

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@@ Line 28: / Line 28: @@
 === NetGO ===
-We used NetGo 2.0 to help visualize the potential function of 1ZBS. This software uses predictive modeling to determine potential functions of the protein in query, and produces a set of (GO) terms that best aligns with the protein's possible function.
+We used NetGo 2.0 to help visualize the potential function of 1ZBS. This software uses predictive modeling to determine potential functions of the protein in query, and produces a set of gene ontology(GO) terms that best aligns with the protein's possible function. It starts with the most general information and builds to become more specific. These results came back in a list form. We then used AmiGo to visualize the connection between the terms. The final results landed us with a protein that has a purine ribonucleotide triphosphate binding domain, and could have some NAG kinase activity.
 ==''' Docking Analysis'''==
@@ Line 49: / Line 49: @@
 == '''Conclusion''' ==
 The goal of this experiment was to determine the function of 1zbs. We did this by creating a standard curve of BSA to compare our curve to our possible substrate NAG. This was done by a series of dilutions and Bradford assays to obtain a dilution factor by comparing it the absorbance levels of BSA. We concluded from our kinase assay results that 1ZBS is not a kinase because the specific activity was 0.074 U/mg.
-This is a sample scene created with SAT to <scene name="/12/3456/Sample/1">color</scene></scene> by Group, and another to make <scene name='95/957643/1zbs_view_2/1'> a transparent representation</scene> of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes.
 </StructureSection>
 == References ==
 <references/>