BASIL2023GV1ZBS: Difference between revisions
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== '''Kinase Assays''' == | == '''Kinase Assays''' == | ||
We used NAG as our final substrate for the 1ZBS kinase assay, but before the kinase assay could be run, a Bradford Assay was needed to determine the concentration of the 1ZBS protein that was over-expressed and purified in the lab. The Bradford Assay gave us the graph below with an R squared value of 0.99, and the equation listed on the graph. | We used NAG as our final substrate for the 1ZBS kinase assay, but before the kinase assay could be run, a Bradford Assay was needed to determine the concentration of the 1ZBS protein that was over-expressed and purified in the lab. The Bradford Assay gave us the graph below with an R squared value of 0.99, and the equation listed on the graph. Because this R squared value is fairly close to 1.0, we decided to use this equation to solved for the concentration of the 1ZBS that we over-expressed. | ||
[[Image:1ZBS_Concentration.png]] | [[Image:1ZBS_Concentration.png]] | ||
After we had run the Bradford Assay, we then ran a kinase assay with 1ZBS and NAG as the substrate. From this assay, we were able to determine that the specific activity was 0.0743 U/mg which is fairly low. | |||
[[Image:1ZBS_CHART.png]] | [[Image:1ZBS_CHART.png]] | ||
== '''Conclusion''' == | == '''Conclusion''' == | ||
