4xih: Difference between revisions

Latest revision as of 10:48, 10 January 2024

Crystal structure of the R116A mutant AhpE from Mycobacterium tuberculosis

Structural highlights

4xih is a 2 chain structure with sequence from Mycobacterium tuberculosis CCDC5079. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.25Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

AHPE_MYCTU Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides. May represent an important antioxidant defense against cytotoxic peroxides, especially peroxynitrite, which can be formed by activated macrophages during infection.^[1] ^[2]

Publication Abstract from PubMed

Peroxiredoxins catalyze the reduction of peroxides, a process of vital importance to survive oxidative stress. A nucleophilic cysteine, also known as the peroxidatic cysteine, is responsible for this catalytic process. We used the Mycobacterium tuberculosis alkyl hydroperoxide reductase E (MtAhpE) as a model to investigate the effect of the chemical environment on the specificity of the reaction. Using an integrative structural (R116A - PDB ; F37H - PDB ), kinetic and computational approach, we explain the mutational effects of key residues in its environment. This study shows that the active site residues are specifically oriented to create an environment which selectively favours a reaction with peroxides.

The active site architecture in peroxiredoxins: a case study on Mycobacterium tuberculosis AhpE.,Pedre B, van Bergen LA, Pallo A, Rosado LA, Dufe VT, Molle IV, Wahni K, Erdogan H, Alonso M, Proft F, Messens J Chem Commun (Camb). 2016 Jul 29. PMID:27471753^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Hugo M, Turell L, Manta B, Botti H, Monteiro G, Netto LE, Alvarez B, Radi R, Trujillo M. Thiol and sulfenic acid oxidation of AhpE, the one-cysteine peroxiredoxin from Mycobacterium tuberculosis: kinetics, acidity constants, and conformational dynamics. Biochemistry. 2009 Oct 13;48(40):9416-26. PMID:19737009 doi:10.1021/bi901221s
↑ Hugo M, Van Laer K, Reyes AM, Vertommen D, Messens J, Radi R, Trujillo M. Mycothiol/mycoredoxin 1-dependent reduction of the peroxiredoxin AhpE from Mycobacterium tuberculosis. J Biol Chem. 2014 Feb 21;289(8):5228-39. PMID:24379404 doi:10.1074/jbc.M113.510248
↑ Pedre B, van Bergen LA, Pallo A, Rosado LA, Dufe VT, Molle IV, Wahni K, Erdogan H, Alonso M, Proft F, Messens J. The active site architecture in peroxiredoxins: a case study on Mycobacterium tuberculosis AhpE. Chem Commun (Camb). 2016 Jul 29. PMID:27471753 doi:https://dx.doi.org/10.1039/c6cc02645a

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OCA

[1] Hugo M, Turell L, Manta B, Botti H, Monteiro G, Netto LE, Alvarez B, Radi R, Trujillo M. Thiol and sulfenic acid oxidation of AhpE, the one-cysteine peroxiredoxin from Mycobacterium tuberculosis: kinetics, acidity constants, and conformational dynamics. Biochemistry. 2009 Oct 13;48(40):9416-26. PMID:19737009 doi:10.1021/bi901221s

[2] Hugo M, Van Laer K, Reyes AM, Vertommen D, Messens J, Radi R, Trujillo M. Mycothiol/mycoredoxin 1-dependent reduction of the peroxiredoxin AhpE from Mycobacterium tuberculosis. J Biol Chem. 2014 Feb 21;289(8):5228-39. PMID:24379404 doi:10.1074/jbc.M113.510248

[3] Pedre B, van Bergen LA, Pallo A, Rosado LA, Dufe VT, Molle IV, Wahni K, Erdogan H, Alonso M, Proft F, Messens J. The active site architecture in peroxiredoxins: a case study on Mycobacterium tuberculosis AhpE. Chem Commun (Camb). 2016 Jul 29. PMID:27471753 doi:https://dx.doi.org/10.1039/c6cc02645a

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 == Structural highlights ==
 <table><tr><td colspan='2'>[[4xih]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_CCDC5079 Mycobacterium tuberculosis CCDC5079]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XIH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4XIH FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4xih FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xih OCA], [https://pdbe.org/4xih PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4xih RCSB], [https://www.ebi.ac.uk/pdbsum/4xih PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4xih ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4xih FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xih OCA], [https://pdbe.org/4xih PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4xih RCSB], [https://www.ebi.ac.uk/pdbsum/4xih PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4xih ProSAT]</span></td></tr>
 </table>
 == Function ==

4xih: Difference between revisions

Latest revision as of 10:48, 10 January 2024

Crystal structure of the R116A mutant AhpE from Mycobacterium tuberculosis

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

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