Sandbox Reserved 1798: Difference between revisions

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The active site is deep within the enzyme. Instead of positively charged side chains contacting the 3-EPB carboxylate groups, they land at the N termini of the α3 and α4 for the benzoate carboxylic group and α1 and α6 for the enol pyruvate group. The negative charges in the groups connect with positive poles on the alphahelices. The acetate ion on DrMqnA connects to strong hydrogen bonds on Asn17 and Ser86. The main amide chain is composed of Gly151.

The 3-EPB benzoate carboxylic group bonds with hydrogen bonds to Thr59 and Ser109. Arg110, Thr111 and Ser112 compose the mobile loop. It is thought that 3-EPB binds and draws residues from lobe 2 to the active site. Two water molecules form hydrogen bonds with the carboxy groups on the 3-EPB.

The 3-EPB benzoate carboxylic group bonds with hydrogen bonds to Thr59 and <scene name='95/954095/Catalytic_triad/2'>Ser109. Arg110, Thr111 and Ser112</scene> compose the mobile loop. It is thought that 3-EPB binds and draws residues from lobe 2 to the active site. Two water molecules form hydrogen bonds with the carboxy groups on the 3-EPB.

Hydrogen bonds form between enol pyruvyl group of 3-EPB during dehydration to position C3 of 3-EPB and the carboxylate oxygen O13 on the enzyme. Hydrogens consequently leave the C3 by the enol pyruvyl group.

Sandbox Reserved 1798: Difference between revisions

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@@ Line 35: / Line 35: @@
 The active site is deep within the enzyme. Instead of positively charged side chains contacting the 3-EPB carboxylate groups, they land at the N termini of the α3 and α4 for the benzoate carboxylic group and α1 and α6 for the enol pyruvate group. The negative charges in the groups connect with positive poles on the alphahelices. The acetate ion on DrMqnA connects to strong hydrogen bonds on Asn17 and Ser86. The main amide chain is composed of Gly151.
-The 3-EPB benzoate carboxylic group bonds with hydrogen bonds to Thr59 and Ser109. Arg110, Thr111 and Ser112 compose the mobile loop. It is thought that 3-EPB binds and draws residues from lobe 2 to the active site. Two water molecules form hydrogen bonds with the carboxy groups on the 3-EPB.
+The 3-EPB benzoate carboxylic group bonds with hydrogen bonds to Thr59 and <scene name='95/954095/Catalytic_triad/2'>Ser109. Arg110, Thr111 and Ser112</scene> compose the mobile loop. It is thought that 3-EPB binds and draws residues from lobe 2 to the active site. Two water molecules form hydrogen bonds with the carboxy groups on the 3-EPB.
 Hydrogen bonds form between enol pyruvyl group of 3-EPB during dehydration to position C3 of 3-EPB and the carboxylate oxygen O13 on the enzyme. Hydrogens consequently leave the C3 by the enol pyruvyl group.