1kou: Difference between revisions

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[[Image:1kou.gif|left|200px]]
{{Seed}}
[[Image:1kou.png|left|200px]]


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{{STRUCTURE_1kou|  PDB=1kou  |  SCENE=  }}  
{{STRUCTURE_1kou|  PDB=1kou  |  SCENE=  }}  


'''Crystal Structure of the Photoactive Yellow Protein Reconstituted with Caffeic Acid at 1.16 A Resolution'''
===Crystal Structure of the Photoactive Yellow Protein Reconstituted with Caffeic Acid at 1.16 A Resolution===




==Overview==
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A structural study is described of the photoactive yellow protein (PYP) reconstituted with the chromophore derivative 3,4-dihydroxycinnamic acid. The crystal structure of PYP reconstituted with this chromophore at 1.16 A resolution is reported in space group P6(5). This is the first high-resolution structure of a photoreceptor containing a modified chromophore. The introduction of an extra hydroxyl group in the native chromophore (i.e. p-coumaric acid) appears to perturb the structure of the hybrid yellow protein only slightly. The chromophore is bound by the protein in two different conformations, separated by a rotation of 180 degrees of the catechol ring. In combination with available spectroscopic data, it is concluded that the caffeic acid chromophore binds to the protein in a strained conformation, which leads to a faster ejection from the chromophore-binding pocket upon pB formation.
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{{ABSTRACT_PUBMED_11914481}}


==About this Structure==
==About this Structure==
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[[Category: Joshua-Tor, L.]]
[[Category: Joshua-Tor, L.]]
[[Category: Photoreceptor]]
[[Category: Photoreceptor]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:59:53 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 10:40:45 2008''

Revision as of 07:40, 2 July 2008

File:1kou.png

Template:STRUCTURE 1kou

Crystal Structure of the Photoactive Yellow Protein Reconstituted with Caffeic Acid at 1.16 A Resolution

Template:ABSTRACT PUBMED 11914481

About this Structure

1KOU is a Single protein structure of sequence from Halorhodospira halophila. Full crystallographic information is available from OCA.

Reference

Structure of the photoactive yellow protein reconstituted with caffeic acid at 1.16 A resolution., van Aalten DM, Crielaard W, Hellingwerf KJ, Joshua-Tor L, Acta Crystallogr D Biol Crystallogr. 2002 Apr;58(Pt 4):585-90. Epub 2002, Mar 22. PMID:11914481

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