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[[Image:1kpd.jpg|left|200px]]
{{Seed}}
[[Image:1kpd.png|left|200px]]


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{{STRUCTURE_1kpd|  PDB=1kpd  |  SCENE=  }}  
{{STRUCTURE_1kpd|  PDB=1kpd  |  SCENE=  }}  


'''A MUTANT RNA PSEUDOKNOT THAT PROMOTES RIBOSOMAL FRAMESHIFTING IN MOUSE MAMMARY TUMOR VIRUS, NMR, MINIMIZED AVERAGE STRUCTURE'''
===A MUTANT RNA PSEUDOKNOT THAT PROMOTES RIBOSOMAL FRAMESHIFTING IN MOUSE MAMMARY TUMOR VIRUS, NMR, MINIMIZED AVERAGE STRUCTURE===




==Overview==
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A single A--&gt;G mutation that changes a potential A.U base pair to a G.U pair at the junction of the stems and loops of a non-frameshifting pseudoknot dramatically increases its frameshifting efficiency in mouse mammary tumor virus. The structure of the non-frameshifting pseudoknot APK has been found to be very different from that of pseudoknots that cause efficient frameshifting [Kang,H., Hines,J.V. and Tinoco,I. (1995) J. Mol. Biol. , 259, 135-147]. The 3-dimensional structure of the mutant pseudoknot was determined by restrained molecular dynamics based on NMR-derived interproton distance and torsion angle constraints. One striking feature of the mutant pseudoknot compared with the parent pseudoknot is that a G.U base pair forms at the top of stem 2, thus leaving only 1 nt at the junction of the two stems. The conformation is very different from that of the previously determined non-frameshifting parent pseudoknot, which lacks the A.U base pair at the top of the stem and has 2 nt between the stems. However, the conformation is quite similar to that of efficient frameshifting pseudoknots whose structures were previously determined by NMR. A single adenylate residue intervenes between the two stems and interrupts their coaxial stacking. This unpaired nucleotide produces a bent structure. The structural similarity among the efficient frameshifting pseudoknots indicates that a specific conformation is required for ribosomal frameshifting, further implying a specific interaction of the pseudoknot with the ribosome.
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{{ABSTRACT_PUBMED_9115361}}


==About this Structure==
==About this Structure==
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KPD OCA].  
Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KPD OCA].  


==Reference==
==Reference==
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[[Category: Ribonucleic acid]]
[[Category: Ribonucleic acid]]
[[Category: Rna pseudoknot]]
[[Category: Rna pseudoknot]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:00:45 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 10:42:30 2008''

Revision as of 07:42, 2 July 2008

File:1kpd.png

Template:STRUCTURE 1kpd

A MUTANT RNA PSEUDOKNOT THAT PROMOTES RIBOSOMAL FRAMESHIFTING IN MOUSE MAMMARY TUMOR VIRUS, NMR, MINIMIZED AVERAGE STRUCTURE

Template:ABSTRACT PUBMED 9115361

About this Structure

Full experimental information is available from OCA.

Reference

A mutant RNA pseudoknot that promotes ribosomal frameshifting in mouse mammary tumor virus., Kang H, Tinoco I Jr, Nucleic Acids Res. 1997 May 15;25(10):1943-9. PMID:9115361

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