|
|
| Line 1: |
Line 1: |
| [[Image:1kr5.gif|left|200px]] | | {{Seed}} |
| | [[Image:1kr5.png|left|200px]] |
|
| |
|
| <!-- | | <!-- |
| Line 9: |
Line 10: |
| {{STRUCTURE_1kr5| PDB=1kr5 | SCENE= }} | | {{STRUCTURE_1kr5| PDB=1kr5 | SCENE= }} |
|
| |
|
| '''Crystal structure of human L-isoaspartyl methyltransferase'''
| | ===Crystal structure of human L-isoaspartyl methyltransferase=== |
|
| |
|
|
| |
|
| ==Overview==
| | <!-- |
| The enzyme l-isoaspartyl methyltransferase initiates the repair of damaged proteins by recognizing and methylating isomerized and racemized aspartyl residues in aging proteins. The crystal structure of the human enzyme containing a bound S-adenosyl-l-homocysteine cofactor is reported here at a resolution of 2.1 A. A comparison of the human enzyme to homologs from two other species reveals several significant differences among otherwise similar structures. In all three structures, we find that three conserved charged residues are buried in the protein interior near the active site. Electrostatics calculations suggest that these buried charges might make significant contributions to the energetics of binding the charged S-adenosyl-l-methionine cofactor and to catalysis. We suggest a possible structural explanation for the observed differences in reactivity toward the structurally similar l-isoaspartyl and d-aspartyl residues in the human, archael, and eubacterial enzymes. Finally, the human structure reveals that the known genetic polymorphism at residue 119 (Val/Ile) maps to an exposed region away from the active site. | | The line below this paragraph, {{ABSTRACT_PUBMED_11792715}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 11792715 is the PubMed ID number. |
| | --> |
| | {{ABSTRACT_PUBMED_11792715}} |
|
| |
|
| ==About this Structure== | | ==About this Structure== |
| Line 29: |
Line 33: |
| [[Category: Yeates, T O.]] | | [[Category: Yeates, T O.]] |
| [[Category: Rossmann-fold doubly-wound-alpha-beta-alpha-sandwich]] | | [[Category: Rossmann-fold doubly-wound-alpha-beta-alpha-sandwich]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:04:22 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 10:49:18 2008'' |
