1kvw: Difference between revisions

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[[Image:1kvw.gif|left|200px]]
{{Seed}}
[[Image:1kvw.png|left|200px]]


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{{STRUCTURE_1kvw|  PDB=1kvw  |  SCENE=  }}  
{{STRUCTURE_1kvw|  PDB=1kvw  |  SCENE=  }}  


'''CARBOXYLIC ESTER HYDROLASE, SINGLE MUTANT H48Q OF BOVINE PANCREATIC PLA2 ENZYME'''
===CARBOXYLIC ESTER HYDROLASE, SINGLE MUTANT H48Q OF BOVINE PANCREATIC PLA2 ENZYME===




==Overview==
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Crystal structures of the active-site mutants D99A and H48Q and the calcium-loop mutant D49E of bovine phospholipase A2 have been determined at around 1.9 A resolution. The D99A mutant is isomorphous to the orthorhombic recombinant enzyme, space group P212121. The H48Q and the calcium-loop mutant D49E are isomorphous to the trigonal recombinant enzyme, space group P3121. The two active-site mutants show no major structural perturbations. The structural water is absent in D99A and, therefore, the hydrogen-bonding scheme is changed. In H48Q, the catalytic water is present and hydrogen bonded to Gln48 N, but the second water found in native His48 is absent. In the calcium-loop mutant D49E, the two water molecules forming the pentagonal bipyramid around calcium are absent and only one O atom of the Glu49 carboxylate group is coordinated to calcium, resulting in only four ligands.
The line below this paragraph, {{ABSTRACT_PUBMED_10089353}}, adds the Publication Abstract to the page
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{{ABSTRACT_PUBMED_10089353}}


==About this Structure==
==About this Structure==
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[[Category: Enzyme]]
[[Category: Enzyme]]
[[Category: Hydrolase]]
[[Category: Hydrolase]]
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