8a53: Difference between revisions

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== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[8a53]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8A53 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8A53 FirstGlance]. <br>
<table><tr><td colspan='2'>[[8a53]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8A53 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8A53 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8a53 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8a53 OCA], [https://pdbe.org/8a53 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8a53 RCSB], [https://www.ebi.ac.uk/pdbsum/8a53 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8a53 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8a53 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8a53 OCA], [https://pdbe.org/8a53 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8a53 RCSB], [https://www.ebi.ac.uk/pdbsum/8a53 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8a53 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[https://www.uniprot.org/uniprot/MCA9_ARATH MCA9_ARATH] Cysteine protease that cleaves specifically after arginine or lysine residues. Does not cleave caspase-specific substrates. Required for proteolytic processing of GRI (PubMed:25398910).<ref>PMID:25398910</ref>  
[https://www.uniprot.org/uniprot/MCA9_ARATH MCA9_ARATH] Cysteine protease that cleaves specifically after arginine or lysine residues. Does not cleave caspase-specific substrates. Required for proteolytic processing of GRI (PubMed:25398910).<ref>PMID:25398910</ref>  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Metacaspases are part of an evolutionarily broad family of multifunctional cysteine proteases, involved in disease and normal development. As the structure-function relationship of metacaspases remains poorly understood, we solved the X-ray crystal structure of an Arabidopsis thaliana type II metacaspase (AtMCA-IIf) belonging to a particular subgroup not requiring calcium ions for activation. To study metacaspase activity in plants, we developed an in vitro chemical screen to identify small molecule metacaspase inhibitors and found several hits with a minimal thioxodihydropyrimidine-dione structure, of which some are specific AtMCA-IIf inhibitors. We provide mechanistic insight into the basis of inhibition by the TDP-containing compounds through molecular docking onto the AtMCA-IIf crystal structure. Finally, a TDP-containing compound (TDP6) effectively hampered lateral root emergence in vivo, probably through inhibition of metacaspases specifically expressed in the endodermal cells overlying developing lateral root primordia. In the future, the small compound inhibitors and crystal structure of AtMCA-IIf can be used to study metacaspases in other species, such as important human pathogens, including those causing neglected diseases.
Structure-function study of a Ca(2+)-independent metacaspase involved in lateral root emergence.,Stael S, Sabljic I, Audenaert D, Andersson T, Tsiatsiani L, Kumpf RP, Vidal-Albalat A, Lindgren C, Vercammen D, Jacques S, Nguyen L, Njo M, Fernandez-Fernandez AD, Beunens T, Timmerman E, Gevaert K, Van Montagu M, Stahlberg J, Bozhkov PV, Linusson A, Beeckman T, Van Breusegem F Proc Natl Acad Sci U S A. 2023 May 30;120(22):e2303480120. doi: , 10.1073/pnas.2303480120. Epub 2023 May 22. PMID:37216519<ref>PMID:37216519</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8a53" style="background-color:#fffaf0;"></div>
== References ==
== References ==
<references/>
<references/>

Latest revision as of 07:56, 1 May 2024

Crystal structure of AtMCA-IIf C147A (metacaspase 9) from Arabidopsis thaliana

8a53, resolution 1.95Å

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