1l2h: Difference between revisions

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[[Image:1l2h.jpg|left|200px]]
{{Seed}}
[[Image:1l2h.png|left|200px]]


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{{STRUCTURE_1l2h|  PDB=1l2h  |  SCENE=  }}  
{{STRUCTURE_1l2h|  PDB=1l2h  |  SCENE=  }}  


'''Crystal structure of Interleukin 1-beta F42W/W120F mutant'''
===Crystal structure of Interleukin 1-beta F42W/W120F mutant===




==Overview==
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The crystal structure at 1.54 A resolution of a double mutant of interleukin-1beta (F42W/W120F), a cytokine secreted by macrophages, was determined by multiple-wavelength anomalous dispersion (MAD) using data from highly twinned selenomethionine-modified crystals. The space group is P4(3), with unit-cell parameters a = b = 53.9, c = 77.4 A. Self-rotation function analysis and various intensity statistics revealed the presence of merohedral twinning in crystals of both the native (twinning fraction alpha approximately 0.35) and SeMet (alpha approximately 0.40) forms. Structure determination and refinement are discussed with emphasis on the possible reasons for successful phasing using untreated twinned MAD data.
The line below this paragraph, {{ABSTRACT_PUBMED_12554939}}, adds the Publication Abstract to the page
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{{ABSTRACT_PUBMED_12554939}}


==About this Structure==
==About this Structure==
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[[Category: Mad phasing]]
[[Category: Mad phasing]]
[[Category: Protein folding]]
[[Category: Protein folding]]
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