1ko9: Difference between revisions

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[[Image:1ko9.gif|left|200px]]<br />

[[Image:1ko9.gif|left|200px]]<br /><applet load="1ko9" size="350" color="white" frame="true" align="right" spinBox="true"

<applet load="1ko9" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

caption="1ko9, resolution 2.15Å" />

'''Native Structure of the Human 8-oxoguanine DNA Glycosylase hOGG1'''<br />

==Overview==

Both 8oxo-guanine and formamidopyrimidines are major products of oxidative, DNA damage that can result in the fixation of transversion mutations, following replication if left unrepaired. These lesions are targeted by, the N-DNA glycosylase hOgg1, which catalyses excision of the aberrant base, followed by cleavage of the phosphate backbone directly 5' to the, resultant abasic site in a context, dependent manner. We present the, crystal structure of native hOgg1 refined to 2.15 A resolution that, reveals a number of highly significant conformational changes on, association with DNA that are clearly required for substrate recognition, and specificity. Changes of this magnitude appear to be unique to hOgg1, and have not been observed in any of the DNA-glycosylase structures, analysed to date where both native and DNA-bound forms are available. It, has been possible to identify a mechanism whereby the catalytic residue, Lys 249 is "primed" for nucleophilic attack of the N-glycosidic bond.

Both 8oxo-guanine and formamidopyrimidines are major products of oxidative DNA damage that can result in the fixation of transversion mutations following replication if left unrepaired. These lesions are targeted by the N-DNA glycosylase hOgg1, which catalyses excision of the aberrant base followed by cleavage of the phosphate backbone directly 5' to the resultant abasic site in a context, dependent manner. We present the crystal structure of native hOgg1 refined to 2.15 A resolution that reveals a number of highly significant conformational changes on association with DNA that are clearly required for substrate recognition and specificity. Changes of this magnitude appear to be unique to hOgg1 and have not been observed in any of the DNA-glycosylase structures analysed to date where both native and DNA-bound forms are available. It has been possible to identify a mechanism whereby the catalytic residue Lys 249 is "primed" for nucleophilic attack of the N-glycosidic bond.

==Disease==

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==About this Structure==

1KO9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1KO9 OCA].

1KO9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KO9 OCA].

==Reference==

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[[Category: Homo sapiens]]

[[Category: Single protein]]

[[Category: Barrett, T.E.]]

[[Category: Barrett, T E.]]

[[Category: Bjoras, M.]]

[[Category: Luna, L.]]

[[Category: Pearl, L.H.]]

[[Category: Pearl, L H.]]

[[Category: Seeberg, E.]]

[[Category: SO4]]

[[Category: helix-hairpin-helix dna-glycosylase]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Mon Nov 12 17~~:52:~~58 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:36:12 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1ko9.gif|left|200px]]<br />
+[[Image:1ko9.gif|left|200px]]<br /><applet load="1ko9" size="350" color="white" frame="true" align="right" spinBox="true"
-<applet load="1ko9" size="450" color="white" frame="true" align="right" spinBox="true"
 caption="1ko9, resolution 2.15&Aring;" />
 '''Native Structure of the Human 8-oxoguanine DNA Glycosylase hOGG1'''<br />
 ==Overview==
-Both 8oxo-guanine and formamidopyrimidines are major products of oxidative, DNA damage that can result in the fixation of transversion mutations, following replication if left unrepaired. These lesions are targeted by, the N-DNA glycosylase hOgg1, which catalyses excision of the aberrant base, followed by cleavage of the phosphate backbone directly 5' to the, resultant abasic site in a context, dependent manner. We present the, crystal structure of native hOgg1 refined to 2.15 A resolution that, reveals a number of highly significant conformational changes on, association with DNA that are clearly required for substrate recognition, and specificity. Changes of this magnitude appear to be unique to hOgg1, and have not been observed in any of the DNA-glycosylase structures, analysed to date where both native and DNA-bound forms are available. It, has been possible to identify a mechanism whereby the catalytic residue, Lys 249 is "primed" for nucleophilic attack of the N-glycosidic bond.
+Both 8oxo-guanine and formamidopyrimidines are major products of oxidative DNA damage that can result in the fixation of transversion mutations following replication if left unrepaired. These lesions are targeted by the N-DNA glycosylase hOgg1, which catalyses excision of the aberrant base followed by cleavage of the phosphate backbone directly 5' to the resultant abasic site in a context, dependent manner. We present the crystal structure of native hOgg1 refined to 2.15 A resolution that reveals a number of highly significant conformational changes on association with DNA that are clearly required for substrate recognition and specificity. Changes of this magnitude appear to be unique to hOgg1 and have not been observed in any of the DNA-glycosylase structures analysed to date where both native and DNA-bound forms are available. It has been possible to identify a mechanism whereby the catalytic residue Lys 249 is "primed" for nucleophilic attack of the N-glycosidic bond.
 ==Disease==
@@ Line 11: / Line 10: @@
 ==About this Structure==
-KO9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1KO9 OCA].
+KO9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KO9 OCA].
 ==Reference==
@@ Line 17: / Line 16: @@
 [[Category: Homo sapiens]]
 [[Category: Single protein]]
-[[Category: Barrett, T.E.]]
+[[Category: Barrett, T E.]]
 [[Category: Bjoras, M.]]
 [[Category: Luna, L.]]
-[[Category: Pearl, L.H.]]
+[[Category: Pearl, L H.]]
 [[Category: Seeberg, E.]]
 [[Category: SO4]]
 [[Category: helix-hairpin-helix dna-glycosylase]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 17:52:58 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:36:12 2008''