5nca

Solution structure of ComGC from Streptococcus pneumoniae

spinqualitylabelsall models 5nca Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5nca is a 1 chain structure with sequence from Streptococcus pneumoniae. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Solution NMR Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function COMGC_STRPN Major component of the type IV-like pilus (T4P) that plays a role in transformation (PubMed:28659339). Transformation pili are dynamically extended and retracted, perhaps thereby promoting DNA uptake and transformation (By similarity). Required for transformation (PubMed:24550320). Required for DNA binding (By similarity).[UniProtKB:P25955][UniProtKB:Q8DN88] Publication Abstract from PubMed Type IV pili are important virulence factors on the surface of many pathogenic bacteria and have been implicated in a wide range of diverse functions, including attachment, twitching motility, biofilm formation, and horizontal gene transfer. The respiratory pathogen Streptococcus pneumoniae deploys type IV pili to take up DNA during transformation. These "competence pili" are composed of the major pilin protein ComGC and exclusively assembled during bacterial competence, but their biogenesis remains unclear. Here, we report the high resolution NMR structure of N-terminal truncated ComGC revealing a highly flexible and structurally divergent type IV pilin. It consists of only three alpha-helical segments forming a well-defined electronegative cavity and confined electronegative and hydrophobic patches. The structure is particularly flexible between the first and second alpha-helix with the first helical part exhibiting slightly slower dynamics than the rest of the pilin, suggesting that the first helix is involved in forming the pilus structure core and that parts of helices two and three are primarily surface-exposed. Taken together, our results provide the first structure of a type IV pilin protein involved in the formation of competence-induced pili in Gram-positive bacteria and corroborate the remarkable structural diversity among type IV pilin proteins. Structure of the competence pilus major pilin ComGC in Streptococcus pneumoniae.,Muschiol S, Erlendsson S, Aschtgen MS, Oliveira V, Schmieder P, de Lichtenberg C, Teilum K, Boesen T, Akbey U, Henriques-Normark B J Biol Chem. 2017 Aug 25;292(34):14134-14146. doi: 10.1074/jbc.M117.787671. Epub , 2017 Jun 28. PMID:28659339 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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