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| [[Image:1l6p.jpg|left|200px]] | | {{Seed}} |
| | [[Image:1l6p.png|left|200px]] |
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| {{STRUCTURE_1l6p| PDB=1l6p | SCENE= }} | | {{STRUCTURE_1l6p| PDB=1l6p | SCENE= }} |
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| '''N-terminal of DsbD (residues 20-144) from E. coli.'''
| | ===N-terminal of DsbD (residues 20-144) from E. coli.=== |
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| ==Overview==
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| Escherichia coli DsbD transports electrons across the plasma membrane, a pathway that leads to the reduction of protein disulfide bonds. Three secreted thioredoxin-like factors, DsbC, DsbE, and DsbG, reduce protein disulfide bonds whereby an active site C-X-X-C motif is oxidized to generate a disulfide bond. DsbD catalyzes the reduction of the disulfide of DsbC, DsbE, and DsbG but not of the thioredoxin-like oxidant DsbA. The reduction of DsbC, DsbE, and DsbG occurs by transport of electrons from cytoplasmic thioredoxin to the C-terminal thioredoxin-like domain of DsbD (DsbD(C)). The N-terminal domain of DsbD, DsbD(N), acts as a versatile adaptor in electron transport and is capable of forming disulfides with oxidized DsbC, DsbE, or DsbG as well as with reduced DsbD(C). Isolated DsbD(N) is functional in electron transport in vitro. Crystallized DsbD(N) assumes an immunoglobulin-like fold that encompasses two active site cysteines, C103 and C109, forming a disulfide bond between beta-strands. The disulfide of DsbD(N) is shielded from the environment and capped by a phenylalanine (F70). A model is discussed whereby the immunoglobulin fold of DsbD(N) may provide for the discriminating interaction with thioredoxin-like factors, thereby triggering movement of the phenylalanine cap followed by disulfide rearrangement.
| | The line below this paragraph, {{ABSTRACT_PUBMED_12033924}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 12033924 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_12033924}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Disulfide bond isomerase protein]] | | [[Category: Disulfide bond isomerase protein]] |
| [[Category: Immunoglobulin-like fold]] | | [[Category: Immunoglobulin-like fold]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:36:06 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 11:49:23 2008'' |
