1lfb: Difference between revisions

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[[Image:1lfb.jpg|left|200px]]
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[[Image:1lfb.png|left|200px]]


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{{STRUCTURE_1lfb|  PDB=1lfb  |  SCENE=  }}  
{{STRUCTURE_1lfb|  PDB=1lfb  |  SCENE=  }}  


'''THE X-RAY STRUCTURE OF AN ATYPICAL HOMEODOMAIN PRESENT IN THE RAT LIVER TRANSCRIPTION FACTOR LFB1(SLASH)HNF1 AND IMPLICATIONS FOR DNA BINDING'''
===THE X-RAY STRUCTURE OF AN ATYPICAL HOMEODOMAIN PRESENT IN THE RAT LIVER TRANSCRIPTION FACTOR LFB1(SLASH)HNF1 AND IMPLICATIONS FOR DNA BINDING===




==Overview==
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The transcription factor LFB1/HNF1 from rat liver nuclei is a 628 amino acid protein that functions as a dimer binding to the inverted palindrome GTTAATN-ATTAAC consensus site. We have crystallized a 99 residue protein containing the homeodomain portion of LFB1, and solved its structure using X-ray diffraction data to 2.8 A resolution. The topology and orientation of the helices is essentially the same as that found in the engrailed, MAT alpha 2 and Antennapedia homeodomains, even though the LFB1 homeodomain contains 21 more residues. The 21 residue insertion is found in an extension of helix 2 and consequent lengthening of the connecting loop between helix 2 and helix 3. Comparison with the engrailed homeodomain-DNA complex indicates that the mode of interaction with DNA is similar in both proteins, with a number of conserved contacts in the major groove. The extra 21 residues of the LFB1 homeodomain are not involved in DNA binding. Binding of the LFB1 dimer to a B-DNA palindromic consensus sequence requires either a conformational change of the DNA (presumably bending), or a rearrangement of the subunits relative to the DNA.
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{{ABSTRACT_PUBMED_8491173}}


==About this Structure==
==About this Structure==
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[[Category: Suck, D.]]
[[Category: Suck, D.]]
[[Category: Transcription regulation]]
[[Category: Transcription regulation]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 23:52:14 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 13:09:25 2008''

Revision as of 10:09, 29 July 2008

File:1lfb.png

Template:STRUCTURE 1lfb

THE X-RAY STRUCTURE OF AN ATYPICAL HOMEODOMAIN PRESENT IN THE RAT LIVER TRANSCRIPTION FACTOR LFB1(SLASH)HNF1 AND IMPLICATIONS FOR DNA BINDING

Template:ABSTRACT PUBMED 8491173

About this Structure

1LFB is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.

Reference

The X-ray structure of an atypical homeodomain present in the rat liver transcription factor LFB1/HNF1 and implications for DNA binding., Ceska TA, Lamers M, Monaci P, Nicosia A, Cortese R, Suck D, EMBO J. 1993 May;12(5):1805-10. PMID:8491173

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